外源性白细胞介素-10对重症急性胰腺炎的肠屏障功能保护作用

目的:探讨外源性白细胞介素-10(IL-10)对大鼠重症急性胰腺炎(SAP)肠屏障功能的影响。方法:90只SD大鼠随机分为假手术组、SAP组I、L-10治疗组,制模12 h后,检测各组血生化指标、血浆内毒素水平、脏器细菌易位率、小肠黏膜细胞间黏附分子-1(ICAM-1)及观察各组胰腺、小肠病理形态学改变。结果:制模12 h后,SAP组血浆内毒素为(144.682±9.584)EU/L、脏器组织细菌易位率为64.6%、小肠黏膜ICAM-1 5.842±0.706、胰腺病理评分10.62±0.60、小肠病损严重。IL-10治疗组上述指标测定值分别为:(103.112±8.124)EU/L(P<0.05),30.6%(P<0.01),4.102±0.668(P<0.05),4.46±0.52(P<0.01)、小肠病损明显减轻。结论:小肠黏膜黏附分子异常表达及炎性介质平衡紊乱,参与SAP鼠肠屏障功能受损。早期应用外源性IL-10能降低小肠黏膜黏附分子表达,抑制炎性介质过度释放,减少肠源性细菌易位,对SAP鼠肠屏障功能有保护作用。

Protection of Exogenous Interleukin-10 on Gut Barrier in Acute Pancreatitis

Objective: To study the effect of exogenous IL-10 on gut barrier in severe acute pancreatitis(SAP).Methods: Ninety SD rats were randomly divided into three groups as the sham-operation group,SAP group and SAP with IL-10 treated group.Tweleve hours after operation,the indices in every group were recorded as following: the biochemical index,plasma endotoxin,organs bacterial translocation rate,ICAM-1 expression in gut mucosa,and the pathologic changes in pancreas and gut.Results: In SAP group,the plasma endotoxin was(144.682±9.584)EU/L,bacterial translocation rate was 64.6%,ICAM-1 expression(by flow cytometry) was 5.842±0.706,pancreas pathology score was 10.62±0.60,gut damage under microscope was severe.In IL-10 treated group,the above indices were significantly decreased as 103.112±8.124(P<0.05),30.6%(P<0.01),4.102±0.668(P<0.05) and 4.46±0.52(P<0.01),respectively,and the gut damage was ameliorated.Conclusion: The abnormal expression of ICAM in gut mucosa and the disorder of inflammatory interleukin both participated in the gut barrier disfunction during SAP.Early thearpy with exogenous IL-10 could alleviate ICAM-1 expression in gut mucosa,prevent inflammatory interleukin's releasing and decrease the bacterial translocation,which suggested a protective effect of IL-10 on gut barrier in SAP rats.