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Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation,New York,USA |
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| Authors: | Adriana Heguy Dacia Dimartino Christian Marier Paul Zappile Emily Guzman Ralf Duerr Guiqing Wang Jonathan Plitnick Alexis Russell Daryl M Lamson Kirsten St George |
| Institution: | NYU Langone Health, New York, New York, USA (A. Heguy, D. Dimartino, C. Marier, P. Zappile, E. Guzman);NYU Grossman School of Medicine, New York (A. Heguy, R. Duerr, G. Wang);New York State Department of Health, Albany, New York, USA (J. Plitnick, A. Russell, D.M. Lamson, K. St. George);State University of New York at Albany, Albany (K. St. George) |
| Abstract: | Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution. |
| Keywords: | COVID-19 2019 novel coronavirus disease coronavirus disease severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 viruses respiratory infections zoonoses genomics sequence analysis amplification artifact |