萎缩性胃炎胃黏膜组织细胞增殖与凋亡的实验研究

目的　研究热盐水灌胃导致的大鼠萎缩性胃炎胃黏膜组织中的凋亡细胞、增殖细胞和bcl 2、Fas表达状态 ,以探讨长期热咸饮食与慢性萎缩性胃炎 (CAG)发生的关系。方法　采用脱氧核糖核酸转移酶介导等缺口末端标记 (TUNEL)技术及免疫组织化学染色技术。细胞凋亡时DNA含量分析采用流式细胞检测技术。结果　 (1 )TUNEL检测细胞凋亡显示 :在热盐水所致的大鼠CAG中凋亡细胞数明显增多 ,在黏膜全层均可见到 ,呈弥漫性分布。CAG中凋亡细胞指数显著高于正常大鼠胃黏膜 (P <0 0 5) ;(2 )免疫组化法检测凋亡相关基因和增殖细胞核抗原 (PCNA)显示 :bcl 2、Fas及PCNA在热盐水灌胃所致的CAG中的表达率显著高于正常胃黏膜 (P <0 0 5) ;热盐水灌胃不同时间大鼠胃黏膜中bcl 2、Fas蛋白表达率随灌胃时间延长而逐渐增加 ,在 4、8、1 2周 (CAG出现 )时bcl 2蛋白表达率分别为 1 2 5 %、1 6 7%、76 5 % ,Fas蛋白表达率分别为 1 8 7%、2 2 2 %、64 7%。 (3)流式细胞检测显示 :CAG时 ,在G0 /G1 峰前可见一个亚G0 /G1 峰 ,也即凋亡细胞峰出现 ,而在正常胃黏膜时未显示亚G0 /G1 峰。结论　热盐水灌胃可导致大鼠胃黏膜组织细胞增殖和细胞凋亡异常 ,其调控基因在热盐水所致的大鼠CAG发生中起重要作用

Experimental study of apoptosis and proliferation of gastric mucosal cells in chronic atrophic gastritis

Objective To study the relationship between long term high salt hot diet and chronic atrophic gastritis (CAG) The expression of apoptosis cells, proliferation cells, bcl 2 and Fas proteins were detected in the gastric mucosa of CAG rates induced by high salt hot water Methods TUNEL technique and immunohistochemical method were used in the experiment Flow cytometry method was used to detect the DNA content of apoptosis cells Results (1) The detection of apoptosis with TUNEL showed that the apoptosis cells in CAG rates induced by high salt hot water were high These cells could be seen in all layers of gastric mucosa, apoptosis index of model rates was higher than that of normal rates( P <0 05) (2) The result of detecting apoptosis gene and proliferating cell nuclear antigen (PCNA) by immunohistochemical method showed that the expression of bcl 2, Fas and PCNA in the CAG rates induced by high salt hot water was much higher than that of normal gastric mucosa ( P <0 05) The expression rates of bcl 2, Fas proteins were higher with time going on After being given high salt hot water intragastrically for 4, 8,12weeks, the bcl 2 protein expression rates were 12 5%, 16 7%, 76 5%, the Fas protein expression rates were 18 7%, 22 2% and 64 7% (3) Flow cytometry technique showed that there was a second G 0/G 1 peek, which was apoptosis cells peak, but was not seen in the normal gastric mucosa Conclusions Abnormality of proliferation and apoptosis in gastric mucosa could be induced by high salt hot water bcl 2, Fas gend played an important role in this course