褪黑素对庆大霉素致急性肾损害的保护作用

目的 探讨褪黑素对庆大霉素所引起的急性肾损害的治疗作用及其机理。方法 通过肌注庆大霉素诱导急性肾损害模型，分别在给予庆大霉素同时和第五天给予褪黑素治疗。检测肾功能、肾组织氧自由基清除酶及肾组织病理学变化，比较各组差异。结果 在两治疗组，血尿素氮、血肌酐、尿NAG酶降低；肾组织超氧化物歧化酶、过氧化氢酶及谷胱甘肽过氧化物酶升高；肾组织氧化应激标志物MDA降低；肾小管病变减轻，尤以早期治疗组效果更佳。结论 褪黑素可能通过其抗氧化作用而减轻庆大霉素的肾毒性损害。

The protective effect of melatonin on gentamycin-induced nephrotoxicity

Objective To investigate the therapeutical effect and possible mechanism of melatonin on gentamycin induced nephrotoxicity in rats.Methods The nephrotoxicitic model of rat was created by injecting gentamycin into muscles of one thigh. At the same time and after five days later,melatonin was given(5mg/kg body weight,i.m) into other thigh. Renal function and oxygen radical scavenger enzymes in renal tissue were measured.Meanwhile, the effect of this agent on renoprotection were estimate by PAS staining.Results In two treated groups by different time,blood urea nitrogen,creatinine and urinary NAG as well as superoxide diamutase(SOD),catalase(CAT),glutathione peroxidase(GSH Px) were much better than those in model group.In rats of model group there was a widespread tubular damage,but in rats in groups of treatment there was a marked reduction in the intensity of bubular damage.Conclusion melatonin has therapeutic effect on rat gentamycin induced nephrotoxicity probably related to improved activity of oxygen radical scavenger enzymes in the kidney.