HPLC测定妇舒液中黄芩苷含量

 目的:采用HPLC测定妇舒液中黄芩苷含量,以控制该制剂的质量。方法:用十八烷基键合硅胶柱分离黄芩苷,流动相为甲醇-4g·L^-1磷酸(50∶50);检测波长278nm。结果:黄芩苷峰与对硝基苯酚峰的分离度为4.5;理论塔板数以黄芩苷峰计算为3800;线性范围20～900ng(r=0.9999);平均回收率为99.88%,RSD=1.54%(n=5)。结论:本法简便、快速,适用于该产品质量分析检验。

Determination of baicalin in Fushuye by HPLC

OBJECTIVE:To domminate the quality of Fushuye,the contents of baicalin in Fushuye was determined by HPLC.METHOD:Baicalin was separated on Spherisorb C18 column and detected at 278 nm,by using methanol-4g.L-1 phosphoric acid (50∶50) as mobile phase.RESULTS:The resolution was 4.5.The number of theoretical plates calculaled for baicalin peak was 3800.The standard curve was linear over the range of 20 ng～900ng,with the correlation coefficient 0.999 9.The average recovery was 99.88% with RSD=1.54%(n=5). CONCLUSION:The method is simple and fast,and can be used for quantitative analysis of Fushuye.