Evaluation of the resistance of blood plasma to oxidative stress by oxidizability of proteins and lipids during metal-catalyzed oxidation |
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Authors: | E M Bekman O A Baranova E V Gubareva L V Shulenina S N Moskvina Yu A Danilogorskaya O A Azizova |
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Institution: | (1) Institute of Physicochemical Medicine, Russian Ministry of Health, Moscow |
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Abstract: | A new approach for the evaluation of oxidizability of proteins and lipids in the same sample of blood plasma was proposed.
We tested a method for evaluation of metal-catalyzed oxidation of fibrinogen by the formation of bityrosine cross-links during
oxidation detected by the increase in fluorescence at 415 nm. A correlation was revealed between parameters of oxidizability
estimated by this marker and carbonyl derivatives (dinitrophenylhydrazine method). Oxidizability of total proteins from whole
plasma was compared with oxidizability of plasma lipids (marker malonic dialdehyde). Study of these parameters in patients
with coronary heart disease showed that the proposed experimental approach allows us to divide the sample into several subgroups
differing in the resistance to oxidative stress. These data can be used for diagnostic and prognostic purposes.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 142, No. 9, pp. 268–272, September, 2006 |
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Keywords: | Cu2+-catalyzed oxidation of lipids and proteins malonic dialdehyde blood plasma oxidative stress |
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