糖尿病大鼠神经功能、形态和半乳糖神经酰胺转移酶的变化

目的 观察早期糖尿病大鼠坐骨神经半乳糖神经酰胺转移酶(CGTmRNA)的变化，及其与神经功能、形态的关系。方法以链脲佐菌素诱导糖尿病大鼠模型，用电生理测定坐骨神经传导速度，光镜、电镜观察坐骨神经形态，半定量RT-PCR方法测定坐骨神经CGTmRNA。结果 糖尿病4周时，坐骨神经传导速度均明显减慢，超微结构改变，而CGTmRNA表达无明显变化；8周时，坐骨神经有明显的形态学改变，CGTmRNA表达明显增高。结论糖尿病多发性神经病(DPN)时坐骨神经CGTmRNA表达上调；其出现于神经功能和形态损伤后。

Function,Morphology and the Expression of UDP-Galactose:Ceramide Galactosyltransferase in the Diabetic Neuropathy Rats

Purpose To observe the functional and morphological change and the expression of UDP-Galactose:Ceramide Galactosyltransferase mRNA in sciatic nerve of diabetic neuropathy rats. Methods Diabetic rat was induced by a single intraperitoneal injection of streptozotocin. Nerve conduction velocity of sciatic nerve was assessed at baseline,2,4 and 8 weeks. Morphology in sciatic nerve was observed under light and electron microscope in diabetes 4 and 8 weeks,and CGT mRNA was measure by RT-PCR at same time. Results Nerve conduction velocity of the sciatic nerve was significantly reduced after 4 weeks of diabetes, abmormal ultrastructure of the sciatic nerve were also observed,but expression of CGTmRNA had no change. By 8 weeks, morphological abnormalities of the sciatic nerve under light microscope was observed, and CGTmRNA expression was increased significantly. Conclusions CGT mRNA level in sciatic nerve of diabetic neuropathy rats was increased, which was occured behind functional and morphological abnormality.