| 慢性乙型肝炎肝组织HBsAg和HBcAg的表达 |
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| 引用本文： | 盛慧萍,杨岩,刘娅,张栩,焦运,李玉芳.慢性乙型肝炎肝组织HBsAg和HBcAg的表达[J].宁夏医学杂志,2009,31(3):198-200. |
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| 作者姓名： | 盛慧萍 杨岩 刘娅 张栩 焦运 李玉芳 |
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| 作者单位： | 宁夏医科大学附属医院,宁夏,银川,750004 |
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| 基金项目： | 宁夏回族自治区自然科学基金 |
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| 摘 要： | 目的探讨慢性乙型肝炎患者肝组织中HBsAg和HBcAg的表达与血清HBV-DNA含量、肝组织中HBV-DNA含量、肝组织病理及临床之间的关系。方法选择慢性乙肝患者83例,检测血清HBV-DNA的含量,并行肝穿刺取肝组织,用免疫组化法检测乙肝患者肝组织中HBsAg和HBcAg的表达并检测肝组织中HBV-DNA的含量,同时行常规肝组织病理染色。结果在慢性乙型肝炎患者肝组织内HBsAg和HBcAg阳性表达率分别为81.9%和53%。HBsAg 98.5%是浆膜型;HBcAg浆膜型占62.8%、胞核型占37.2%,浆膜型的肝组织炎症、纤维化较胞核型明显。HBsAg表达强度与血清HBV-DNA定量、肝组织HBV-DNA定量及肝组织炎症和纤维化之间均无相关性。HBcAg表达强度随着血清HBV-DNA定量的增加而增加,呈正向关联（r=0.291,P=0.008）,与肝组织HBV-DNA定量呈现明显的正向关联（r=0.664,P〈0.001）,与肝组织炎症、纤维化分级也呈正相关（r=0.611,0.841,P〈0.001）。结论慢性乙肝患者肝组织免疫损伤发生的免疫应答的主要抗原是HBcAg。将肝组织中HBcAg的表达结合HBV-DNA载量作为抗病毒治疗指标更可靠。
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| 关 键 词： | 慢性乙型肝炎 HBV-DNA 表面抗原 核心抗原 炎症 纤维化 免疫组织化学 |
Expression and clinical significance of HBsAg and HBcAg in hepatocytes in the patients with chronic hepatitis B |
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| SHENG Hui-ping,YANG Yan,LIU Ya,et al..Expression and clinical significance of HBsAg and HBcAg in hepatocytes in the patients with chronic hepatitis B[J].Ningxia Medical Journal,2009,31(3):198-200. |
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| Authors: | SHENG Hui-ping YANG Yan LIU Ya |
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| Institution: | The Affiliated Hospital of Ningxia Medical University;Yinchuan 750004;China |
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| Abstract: | Objective To study the expression of HBsAg and HBcAg in hepatocytes in patients with chronic hepatitis B,and to analyze the correlation among the expression of HBsAg and HBcAg,the quantity of HBV DNA in serum and in the liver tissue,the pathology of liver tissue and the clinical manifestation.Methods The polymerase chain reaction was used to exam the quantity of HBV DNA in serum and the fluorescence quantitative polymerase chain reaction for detection of HBV DNA in liver tissues.And the expression of HBsAg ... |
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| Keywords: | HBV-DNA |
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