| 固体垃圾和渗沥液中总DNA提取及微生物多样性分析 |
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| 引用本文： | 陈朱蕾,曹丽,江娟,刘婷,吕志中.固体垃圾和渗沥液中总DNA提取及微生物多样性分析[J].环境卫生工程,2007,15(5):35-38. |
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| 作者姓名： | 陈朱蕾 曹丽 江娟 刘婷 吕志中 |
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| 作者单位： | 华中科技大学,环境科学与工程学院,湖北,武汉,430074 |
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| 基金项目： | 湖北省武汉市科技攻关项目 |
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| 摘 要： | 基于对生活垃圾及其渗沥液提取总DNA方法的评价,采用ARDRA和RISA分析固体垃圾和渗沥液的生物多样性.通过总DNA浓度、纯度、片段分布情况、PCR扩增等指标评价,找出效果较好的提取方法,该法从固体垃圾和渗沥液中均获得了高质量的总DNA,DNA分子片段在23 kb左右,且样品不需经过纯化可以直接进行PCR扩增;ARDRA和RISA分析显示固体垃圾比渗沥液具有更丰富的微生物多样性.
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| 关 键 词： | 固体垃圾 渗沥液 DNA提取 微生物多样性 固体垃圾 渗沥液 提取 微生物多样性 多样性分析 Analysis Leachate Solid Waste DNA Extraction 显示 纯化 样品 分子片段 质量 效果 指标评价 分布情况 浓度 RISA ARDRA |
| 文章编号： | 1005-8206（2007）05-0035-04 |
| 收稿时间： | 2007-05-22 |
| 修稿时间： | 2007年5月22日 |
Total DNA Extraction from Solid Waste and Leachate and Analysis of Their Microbial Diversities |
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| Chen Zhulei,Cao Li,Jiang Juan,Liu Ting,Lü Zhizhong.Total DNA Extraction from Solid Waste and Leachate and Analysis of Their Microbial Diversities[J].Environmental Sanitation Engineering,2007,15(5):35-38. |
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| Authors: | Chen Zhulei Cao Li Jiang Juan Liu Ting Lü Zhizhong |
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| Abstract: | Based on evaluating the methods of total DNA extraction from solid waste and leachate, microbial diversities of solid waste and leachate were analyzed using ARDRA and RISA methods. Better extraction method was found by comparison with PCR-amplification, the concentration, purity, molecular size distribution of the total DNA. Through this method, high quantity total DNA can be extracted. Molecular size of DNA obtained was about 23 kb and the samples could directly be used to PCR-amplification without purification. The analysis results of ARDRA and RISA showed that, the solid waste had the higher microbial diversity compared with leachate. |
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| Keywords: | solid waste leachate DNA extraction microbial diversity |
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