大鼠主要水溶性晶体蛋白在老化进程中的时相变化

目的: 研究大鼠老化进程中主要晶体蛋白相对含量的时相变化与晶体老化的关系。方法: 常规饲养不同年龄SD大鼠(出生后1 d、8 d、2周、8周、8个月及1.5年);提取水溶性晶体蛋白,双向电泳(等电聚焦/SDS-聚丙烯酰胺凝胶电泳, IEF/SDS-PAGE)得到大鼠晶体蛋白电泳图形;考马斯亮蓝染色后扫描蛋白图形,分别确认各主要晶体蛋白组分并测定其相对含量百分比组成。 结果:(1)所测各组大鼠18种主要晶体蛋白组分中,7种晶体蛋白相对含量呈渐进性时相改变,但总水溶性晶体蛋白水平未见明显变化。(2)晶体蛋白相对含量时相改变主要有4种形式:升高(βB₄、αB₂、αA₂、βA₁)、降低(β₇、β₈、γ_2,3、γ_5,6)、大致保持稳定(βA₃、βB₅)及无规则性改变。(3)晶体蛋白βB₄ /αA₂比值在大鼠老化进程中呈渐进性升高。结论: 晶体蛋白相对含量的渐进性时相改变反映晶状体老化的程度。

Phasic change of water soluble major crystallins with aging in rats

AIM: To study the correlation between phasic change of the relative quantity of major crystallins with aging in rats. METHODS: Six groups of SD rats (age 1 d， 8 d，2 weeks，8 weeks，8 months and 1.5 years) were raised routinely. Water soluble crystallins were extracted and separated by two－dimensional polyacrylamide gelelectrophoresis. After comassize blue staining，the crystallins patterns were scanned and analyzed. RESULTS: (1) Out of the eighteen water soluble major rat crystallins tested in each group, seven showed gradual phasic changes in relative quantity of crystallins, but there were no significant changes in total quantity of water soluble crystallins. (2) Phasic changes in these crystallins presented four different patterns: increasing (βB₄、αB₂、αA₂、βA₁), decreasing (β₇、β₈、γ_2,3、γ_5,6),relatively stable(βA₃、βB₅), and irregular. (3) The ratio of βB₄ /αA₂ increased gradually with the rat aging process. CONCLUSION: The gradual phasic changes in relative quantity of crystallins reflect the aging status of rat crystalline.