溶菌酶的反胶束提取条件优化

目的:优化反胶束法分离和提取溶菌酶的条件,为溶菌酶的工业提取提供参考。方法:以溶菌酶质量浓度为评价指标,通过单因素试验和正交试验考察反胶束团中溴化十六烷基三甲铵(CTAB)浓度、正辛烷与正己醇的配比、氯化钾溶液浓度、被萃取液与萃取液比例对溶菌酶提取工艺的影响。结果:溶菌酶的最优提取条件为CTAB浓度30 mmol·L-1,正辛烷-正己醇(4∶1),KCl浓度0.15 mol·L-1,被萃取液-萃取液(1∶2)。在该条件下,溶菌酶质量浓度1.330 g·L-1。结论:反胶束溶液提取溶菌酶的方法具有液液萃取的优点,溶菌酶不容易变性失活且溶解度较高,值得进一步推广与开发。

Optimization of Reverse Micelles Extraction Conditions of Lysozyme

Objective: To optimize separation and extraction method of lysozyme by reversed micellar solution. Method: Taking the content of lysozyme as index, orthogonal test and single factor tests were adopted to optimize extraction process with CTAB concentration in reversed micellar clusters solution, ratio of N-octane and n-hexanol, KCl solution concentration and extract liquid to being extract liquid ratio as factors. Result: Optimum extraction conditions of lysozyme were as follows:CTAB concentration of 30 mmol·L^-1, ratio of N-octane and n-hexanol of 4:1, KCl concentration of 0.15 mol·L^-1, extract liquid-being extracted liquid (1:2).The content of lysozyme was 1.330 g·L^-1. Conclusion: Extracting lysozyme by reversed micellar solution has advantages of liquid-liquid extraction, while lysozyme is not deactivated and its solubility is higher, this technology is worthy further development.