不同产地栀子药材中8种主要药效成分的含量测定及聚类分析

目的:比较不同产地栀子药材中京尼平苷酸、去乙酰车叶草酸甲酯、京尼平龙胆双糖苷、栀子苷、新绿原酸、绿原酸、隐绿原酸及西红花苷Ⅰ共8种药效成分的含量,为栀子药材最佳产地的确定提供理论依据。方法:采用HPLC测定各指标成分含量,流动相乙腈(A)-0.1%磷酸溶液(B)梯度洗脱(0~10 min,5%~10%A;10~20 min,10%~18%A;20~30 min,18%~45%A,40~50 min,45%~5%A),检测波长分别238,324,440 nm。并以各成分作为指标进行聚类分析,采用SPSS 20.0中主成分分析法提取4个主因子。结果:累计方差贡献率95.98%,聚类分析结果显示江西泰和县和江西樟树县GAP基地的栀子药材质量较好。结论:不同产区栀子药材中主要药效成分的含量有较大差异,各成分含量与产地呈一定的相关性。

Determination and Cluster Analysis of Eight Effective Components in Gardeniae Fructus from Different Localities

Objective: To compare contents of eight medicinal components (geniposidic acid, deacetyl asperulosidic acid methyl ester, genipin 1-gentiobioside, gardenoside, crocin I, neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid) in Gardeniae Fructus from different localities, and provide a reference for selecting its optimal locality. Method: HPLC was performed on a Kromasil C₁₈ column with mobile phases of acetonitrile-0.1% phosphoric acid solution in a gradient mode at a flow rate of 1.0 mL·min^-1 and detection wavelength was 238, 324, 440 nm.Taking each component as indeses for cluster analysis, four main factors were extracted by principal component analysis in SPSS 20.0. Result: Four principal component which accounted for over 95% of the total variance, qualities and uniformities of Gardeniae Fructus from GAP bases of Zhangshu county and Taihe county were higher than others. Conclusion: Contents of medicinal components in Gardeniae Fructus from different localities are significant different.There is correlation between contents of each component and localities.