半夏泻心汤治疗胃炎的物质基础及HPLC含量测定研究

目的:初步筛选半夏泻心汤治疗胃炎的药效物质基础,并建立药效物质基础的含量测定方法。方法:采用大孔吸附树脂法(洗脱溶剂分别为水,30%乙醇,70%乙醇)获得半夏泻心汤水煎液的3个分离部位;取健康Wistar大鼠48只,雌雄各半,随机分为6组,每组8只,分别为正常组,模型组,水煎液组(半夏泻心汤水煎液),水洗脱液组(以下简称蒸馏水组),30%乙醇洗脱液组(以下简称30%组),70%乙醇洗脱液组(以下简称70%组),除正常组外,按5 m L·kg-1的剂量以无水乙醇ig建立急性胃炎的实验动物模型,另取健康的成年Wistar大鼠60只,雌雄各半,随机分为6组,每组10只,分组同上,除正常组外,其余5组采用综合造模法来建立大鼠慢性胃炎的模型,对比各分离部位对实验性急、慢性胃炎的治疗作用。结果:70%乙醇洗脱液对胃炎的疗效较好,胃黏膜表面呈淡粉色且较光滑,无明显的溃疡点,溃疡抑制率与模型组比较有显著性差异(P0.05)。采用HPLC法对70%乙醇分离部位中的盐酸小檗碱、黄芩苷进行了含量测定。结论:半夏泻心汤的药效活性物质大多存在于大孔吸附树脂70%乙醇洗脱部位,其治疗胃炎的活性成分与生物碱、黄酮类有关。

Basic Active Components in Banxia Xiexin Tang for Treatment of Gastritis and Determination by HPLC

Objective: To preliminarily screen out the pharmacodynamic material basis of Banxia Xiexin Tang in the treatment of gastritis, and establish the content determination method for the pharmacodynamic material basis. Method: Macroporous adsorption resin chromatography (eluting solvent:water, 30% ethanol, 70% ethanol) was adopted to separate three parts of Banxia Xiexin Tang liquid. Totally 48 healthy Wistar rats (half male and half female) were randomly divided into six groups:the normal group, the model group, the Banxia Xiexin Tang group, the water elution group (the distilled water group for short), the 30% ethanol elution group (the 30% group for short) and the 70% ethanol elution group (the 70% group for short). Except for the normal group, the experimental animal model of acute gastritis was established with absolute ethyl alcohol with the dosage of 5 mL·kg^-1, ig. In addition, 60 healthy Wistar rats (half male and half female) were randomly divided into six groups, with 10 in each group, the same as above. Except for the normal group, the remaining five groups were included in the chronic gastritis model by using the comprehensive modeling method, in order to compare the therapeutic effect of the separate parts in treating experimental acute and chronic gastritis. Result: A better therapeutic effect in treatment of gastritis was found in 70% ethanol elution liquid;the gastric mucosal surface was pale pink and smooth, with no obvious ulcer, with a significant difference in ulcer inhibition rate in comparison with the model group (P<0.05). The content of baicalin and berberine hydrochloride were determined by HPLC method in 70% ethanol elution parts. Conclusion: The efficacy of active substance of Banxia Xiexin Tang mostly existed in the 70% ethanol elution parts separated by macroporous adsorption resin, its active component for treating gastritis are related to alkaloids, flavonoids.