葡萄球菌735株分离鉴定和耐药性分析

目的对临床分离出的葡萄球菌的耐药情况进行统计分析,为有效控制致病性葡萄球菌,减少细菌耐药性提供参考,为经验用药提供有效依据。方法从医院患者的标本中分离出735株葡萄球菌,按美国国家临床实验室标准委员会（NCCLS）的标准,采用纸片琼脂扩散法进行药敏试验。结果葡萄球菌的耐药性均比较强,特别是耐甲氧西林的金黄色葡萄球菌（MRSA）和耐甲氧西林的凝固酶阴性葡萄球菌（MRSCN）,但在MRSCN中,对氨苄西林、阿莫西林/克拉维酸钾的耐药性较低,分别为44.1%、23.0%,低于MRSA。结论葡萄球菌是造成感染的主要病原菌之一,合理使用抗菌药物对于有效治疗疾病和控制医院感染的发生至关重要,葡萄球菌耐药性监测对指导临床用药有重要意义。

Separate identification and analysis of drug resistance on 735 strain staphylococcal

Objective To statistical analysis the information of staphylococal resistance of isolated from the clinical,to offer that valid control the pathogenicity staphylococci,to decrease bacterial drug resistance and provide the effectiveness basis for experience medication.Methods Separated 735 strain staphylococcus from the hospital,according to national committee for clinical laboratory standards（NCCLS）,applied scrip agar diffusion method to drug sensitive test.Results Resistance of staphylococcus was stronger,especially methicillin-resistant Staphylococcus aureus（MRSA）and methicillin-resistant coagulase-negative staphylococci（MRSCN）;But in MRSCN,the pairs of ampicillin,amoxicillin/potassium clavulanate resistance（44.1%,23.0%）were lower lower than MRSA.Conclusion Staphylococcus aureus is one of the main pathogens causing infection,rational use of antimicrobial agents for the effective treatment of disease and the occurrence of nosocomial infection control is essential,staphylococcus aureus resistance surveillance in clinical medicine has an important significance.