| 应用荧光原位杂交技术检测男性肺癌细胞性染色体数目的改变 |
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| 引用本文： | 谢菁,王庆才,陶敏.应用荧光原位杂交技术检测男性肺癌细胞性染色体数目的改变[J].苏州大学学报(自然科学版),2005,25(6):1051-1053. |
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| 作者姓名： | 谢菁 王庆才 陶敏 |
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| 作者单位： | 苏州大学附属第一医院肿瘤科,江苏苏州215006 |
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| 摘 要： | 目的 应用荧光原位杂交技术（FIsH）研究肺癌细胞性染色体数目的改变及其意义。方法 用X、Y号染色体的着丝粒探针与17例男性肺癌标本的间期细胞进行杂交检测。结果男性患者中，X染色体的获得占10例（58．82％），Y染色体的丢失占9例（52．94％），Y染色体的获得占2例。结论 FISH技术可检测肺癌间期细胞的染色体数目改变，性染色体数目异常在肺癌中的发生率很高，与肺癌的发生、发展有一定的联系。
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| 关 键 词： | 肺癌 染色体 荧光原位杂交 男性 |
| 文章编号： | 1673-0399(2005)06-1051-03 |
| 收稿时间： | 2005-04-27 |
| 修稿时间： | 2005年4月27日 |
Detection of Sex Chromosome Numerical Abnormalities in Lung Cancer by Fluorescence in Situ Hybridization (FISH) |
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| XIE Jing, WANG Qing-cai, TAO Min.Detection of Sex Chromosome Numerical Abnormalities in Lung Cancer by Fluorescence in Situ Hybridization (FISH)[J].Suzhou University Journal of Medical Science,2005,25(6):1051-1053. |
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| Authors: | XIE Jing WANG Qing-cai TAO Min |
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| Institution: | Dept of Oncology, the First Hospital Affiliated to Suzhou University, Jiangsu Suzhou 215006, China |
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| Abstract: | Objective To study the numerical abnormality of chromosomes in lung cancer by dual-color fluorescence in situ hybridization(FISH).Methods Seventeen male fresh lung cancer specimens were detect by FISH with the centromeric probes for chromosome X and Y.Results In male,increased chromosome X copy number was observed in 58.82%(10/17) specimens,in 52.94%(9/17) specimens chromosome Y lost,in 2 specimens chromosome Y was gained.Conclusion FISH can detect the chromosomal changes of lung cancer.The gain of chromosome X and the loss of chromosome Y frequently occurred in lung cancer.Thus it can provide the new scientific evidences for early occurrence and development of lung cancer. |
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| Keywords: | lung cancer chromosome fluorescence in situ hybridization(FISH) |
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