滇黄芩茎叶乙醇提取物及其不同溶剂萃取部位的抗氧化和降脂活性研究

目的:研究滇黄芩茎叶乙醇提取物及其不同溶剂萃取部位的抗氧化活性和降脂活性.方法:取滇黄芩茎叶用95％乙醇回流提取,得乙醇提取物;取上述提取物,依次用石油醚、乙酸乙酯、正丁醇萃取,回收溶剂得到不同溶剂萃取部位.以维生素C(Vc)为阳性对照,采用羟基自由基、超氧阴离子自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基...

Study on Antioxidant Activity and Lipid-lowering Effect of Ethanol Extract and Its Different Solvent Extracts from the Stem and Leaves of Scutellaria amoena

OBJECTIVE:To study the antioxidant activity and lipid-lowering effect of ethanol extract and its different solvent extracts from the stems and leaves of Scutellaria amoena.METHODS:The stem and leaves of S.amoena was extracted with 95%ethanol to obtain ethanol extract,and then extracted with petroleum,ethyl acetate and n-butanol to obtain corresponding different solvent extracts.Using vitamin C(Vc)as positive control,the antioxidant activities of ethanol extract,petroleum ether extract,ethyl acetate extract and n-butanol extract from the stems and leaves of S.amoena were determined by hydroxyl radical,superoxide anion radical and DPPH radical scavenging method,and the IC50 was calculated.Steatosis L02 hepatocyte model was established with fat emulsion.Using fenofibrate(20μg/mL)as positive control,the effects of high and low concentration(100 and 50μg/mL)ethanol extract,ethyl acetate extract and n-butanol extract from the stems and leaves of S.amoena on the contents of TC and TG in cells were investigated.RESULTS:The order of scavenging ability to hydroxyl radicals was n-butanol extract>ethyl acetate extract>Vc>ethanol extract>petroleum ether extract;IC50 of them were 0.15,0.17,0.35,0.75,1.17 mg/mL,respectively.The order of scavenging ability to superoxide anion radical was Vc>n-butanol extract>ethyl acetate extract>ethanol extract>petroleum ether extract;IC50 of them were 0.034,0.55,0.75,3.32,3.73 mg/mL,respectively.The order of DPPH scavenging ability to DPPH radical was Vc>n-butanol extract>ethyl acetate extract>ethanol extract>petroleum ether extract;IC50 of them were 0.0032,0.028,0.033,0.048,0.057 mg/mL,respectively.The ethanol extract,ethyl acetate extract and n-butanol extract from the stems and leaves of S.amoena could significantly decrease the contents of TC and TG in steatosis L02 hepatocytes(P<0.01).The order of lipid-lowering ability was n-butanol extract(low dose)≈fenofibrate>ethyl acetate extract(high dose)>ethanol extract(high dose)>n-butanol extract(high dose)>ethyl acetate extract(low dose)>ethanol extract(low dose).CONCLUSIONS:The ethanol extract,petroleum ether extract,ethyl acetate extract and n-butanol extract from the stems and leaves of S.amoena show good antioxidant activity and lipid-lowering effect(except for petroleum ether extract).Ethyl acetate extract and n-butanol extract possess the strongest antioxidant activity and lipid-lowering effect.