甘草饮片中抗氧化活性成分的快速筛选及鉴定

目的:建立在线检测甘草饮片中抗氧化活性成分的方法,并对其进行鉴定.方法:通过检测1,1-二苯基-2-三硝基苯肼(DPPH)清除率来评价甘草饮片的抗氧化活性;采用在线高效液相色谱-紫外-1,1-二苯基-2-三硝基苯肼法(HPLC-UV-DPPH)筛选甘草饮片中的抗氧化活性成分;采用高效液相色谱-飞行时间高分辨质谱法(HP...

Rapid Screening and Identification of Antioxidant Active Components in Glycyrrhiza uralensis Decoction Pieces

OBJECTIVE:To establish a method for online detection of antioxidant active components in Glycyrrhiza uroalensis decoction pieces,and to identify it.METHODS:The free radical scavenging rate of 1,1-diphenyl-2-trinitrobenzene hydrazine(DPPH)was determined to evaluate the antioxidant activity of G.uralensis decoction pieces.HPLC-UV-DPPH method was used to screen the antioxidant active components of G.uralensis decoction pieces.HPLC-TOF/MS was used to obtain mass spectrum data and Qualitive Analyst B 06.00 Build 6.0.633.0 software was used to analyze data.Through contrast analysis of UV absorption spectrum,online chromatogram,mass spectrum information of G.uralensis and the retention time of each compound,accurate molecular weight,antioxidant active components were identified by referring to relevant literature.Validation test was also conducted.RESULTS:DPPH free radical scavenging rate in 8 batches of G.uralensis decoction pieces ranged 55.71%-60.17%.Seven antioxidative active compounds,including avolomotor,8-isopentenyl naringin,yellow lupulin weitone,isoflavone B,3′,4′-dimethoxy3-hydroxy-6-methyl flavone,glycyrrhizin E and glycyrrhizin H,could be screened from G.uralensis decoction pieces.After validation,the peak area of inverted peak generated by online reaction was positively correlated with DPPH free radical scavenging rate.CONCLUSIONS:Established method is simple and accurate,and can be used to quickly screen and identify the main antioxidant components of G.uralensis decoction pieces;the peak area of inverted peak can be used to evaluate the antioxidant active components of G.uralensis decoction pieces.