不同基原八爪金龙药材中黄酮、香豆素类化学成分分析

目的:鉴定并分析不同基原八爪金龙药材中黄酮及香豆素类成分.方法:采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(UPLC-QE-HF-MS/MS).色谱柱为Zorbax Eclipse-C18,流动相为乙腈-0.1％甲酸水溶液(梯度洗脱),柱温为30℃,流速为0.3 mL/min,自动进样器温度为4℃,进样量为2...

Analysis of Chemical Constituents as Flavonoids and Coumarins in Radix Ardisiae from Different Sources

OBJECTIVE:To identify and analyze the flavonoids and coumarins in Radix Ardisiae from different sources.METHODS:UPLC-QE-HF-MS/MS was adopted.The determination was performed on Zorbax Eclipse-C18 column with mobile phase consisted of acetonitrile-0.1%formic acid solution(gradient elution)at the flow rate of 0.3 mL/min.The column temperature was 30℃,and the temperature of injector was 4℃.The sample size was 2μL;ESI source was applied in negative and positive scanning ion mode,the heater temperature was 325℃,the sheath gas pressure was 45 arb,the auxiliary gas pressure was 15 arb,the purge gas pressure was 1 arb,the electrospray voltage was 3.5 kV,the capillary temperature was 330℃,S-lens RF level was 55%,scan mode was first-order full scan m/z 100-1500,data-dependent secondary mass spectrometry scanning(dd-MS2,Top N=10),the resolution was 70000(first mass spectrometry),17500(secondary mass spectrometry),the collision mode was high-energy collision dissociation.Through retrieving foreign and domestic databases as ChemSpider,mzCloud,mzVault,PubChem,the structure of the compound was identified on the basis of related literatures and reference data,and the contents were compared.RESULTS&CONCLUSIONS:A total of 47 components were separated from Radix Ardisiae of 3 kinds of sources as Ardisia crenata Sims,A.crispa(Thunb.)A.DC.,A.crenata Sims var.bicolor(Walk)C.Y.Wu et C.Chen.A total of 17 flavonoids were identified,including 9 flavonols(quercetin 3-O-rhamnoside-7-O-glucoside,myricetin,rutin,mauritanin,kaempferol,quercetin,isorhamnetin,quercetin,mearnsitrin),3 flavan-3-ols[(-)-epigallocatechin,catechin,epigallocatechin gallate)2 dihydroflavonoids[fustin,eriodictyol]and 3 other types[3-(2,3-dihydro-benzo[1,4]dioxin-6-yl)-7-hydroxy-2-trifluoromethyl-chromen-4-one,methadone,oriciacridone F],10 coumarins{bergenin,[(7-hydroxy-4-methyl-2-oxo-2H-chromen-6-yl)oxy]acetic acid,[7-(carboxymethoxy)-4-methyl-2-oxo-2hydroxychromo-3-yl]acetic acid,4,9-dihydroxy-7H-furo[3,2-g]chromen-7-one,6,7-dihydroxy-4-methylcoumarin,esculetin,fraxetin,7,8-dihydroxy-4-methylcoumarin,4-methylumbelliferyl glucuronide,scoparone}.Results of content analysis showed that in flavonoids and coumarins,there were 5 common components in Radix Ardisiae from 3 kinds of sources,i.e.bergenin(peak 2),[7-(carboxymethoxy)-4-methyl-2-oxo-2-hydroxychromo-3-yl]acetic acid(peak 5),methadone(peak 16),quercetin(peak 18),oriciacridone F(peak 26);the contents of common components were significantly different.In addition to 5 common components,there were 22 different chemical components,which were compounds corresponding to peaks 1,3,4,6-15,17,19-25 and 27,respectively.Among them,compounds corresponding to peaks 3,6,8 and 23 were only found in A.crenata Sims var.bicolor(Walk)C.Y.Wu et C.Chen;compounds corresponding to peaks 12-15,19 were only found in A.crispa(Thunb.)A.DC.UPLC-QE-HF-MS/MS method can efficiently,accurately and quickly identify the flavonoids and coumarins in Radix Ardisiae from different sources.