SDF-1的表达与造血细胞回巢关系的实验研究

目的 探讨趋化蛋白SDF－1在造血细胞回巢中的作用。方法 采用异基因小鼠骨髓细胞移植动物模型和免疫组化双染色方法，观察趋化蛋白SDF－1的表达与回巢细胞的关系。结果 骨髓内SDF－1主要表达在骨内膜旁、微血管内皮、骨细胞内和供体阳性细胞周围，与非照射组相比，照射预处理组骨髓中的SDF－1在骨细胞中、骨内膜旁有明显高表达，提示放射预处理可能促使髓内各种基质细胞分泌SDF－1，SDF－1与回巢细胞的关系密切。结论 照射预处理可能是增加骨髓基质细胞分泌SDF－1的激发因素之一，SDF－1在造血细胞回巢中有重要作用。

Experinmental study on relationship between expression of SDF-1 and homing of hematopoietic cells

Objective To investigate the role of chemoattractant SDF 1 in of homing hematopoietic cells. Methods A mouse allogeneic bone marrow transplantation model and double staining of immunohistochemistry were used in this study.Relationship between expression of chemoattractant SDF 1 and of homing hematopoietic cells was observed. Results In bone marrow,SDF|1 was mainly expressed at sites near endosteum,in microvessel endothelium,osteocyte and around donor cells.Contrast to non|irradiation group,SDF|1 was significantly expressed by osteocytes,and at sites near endosteum in the irradiation group, suggesting that pretreatment with irradiation might enhance secretion of SDF|1 by bone marrow stromal cells and SDF|1 could have a close relation with homing cells. Conclusion Pretreatment with irradiation might be one of the stimulating factors significantly increasing the level of SDF|1 produced by bone marrow stromal cells and the chemoattractant SDF|1 could play an important role in homing of hematopoietic cells.;