黄芪总皂苷对完全弗氏佐剂诱导的慢性病贫血模型大鼠膜铁转运蛋白1的影响

目的:探讨黄芪总皂苷(total saponins of astragalus,TSA)对完全弗氏佐剂(complete Freund's adjuvant,CFA)诱导的慢性病贫血(anemia of chronic disease,ACD)模型大鼠膜铁转运蛋白1(ferroportin1,Fpn1)的影响.方法:经大鼠右后足皮内注射含卡介苗10 g·L-1的CFA复制ACD模型,以促红细胞生成素(促红素)为对照,经ip给予TSA(50,16,5 mg·kg-1)连续7d后,采集标本,采用酶联免疫吸附法(ELISA)测定血清白介素-6(IL-6),实时定量逆转录聚合酶链反应法(Quantitative realtime Polymerase Chain Reaction,qPCR)分析大鼠肝、脾、十二指肠组织膜铁转运蛋白(Fpn)及肝组织肝源性杀菌蛋白(Hepatic bactericidal protein,Hepcidin)基因(Hamp mRNA).结果:ACD模型大鼠血清IL-6、肝及十二指肠组织Fpn1、肝组织Hamp mRNA明显上升(P＜0.05),脾组织Fpn1 mRNA没有明显变化;高、中剂量TSA均有降低血清IL-6的作用(P＜0.05),与阳性药物作用相当,低剂量TSA作用不显著;不同剂量TSA均能下调肝及十二指肠组织Fpn1、肝组织Hamp mRNA(P ＜0.05),并上调脾组织Fpn1 mRNA(P ＜0.05),且优于阳性药物(P＜0.05).结论:IL-6引起的Hamp mRNA的升高并未引起相应组织中Fpn1 mRNA的减少,反而在CFA诱导的ACD大鼠肝及十二指肠组织中明显上升,在脾组织中无明显变化;TSA对不同组织中Fpn可能有不同的调节作用.

Effect of Total Saponins of Astragalus on Ferroportin 1 in Rats with Anemia of Chronic Disease Induced by Complete Freund's Adjuvant

Objective:To investigate the effect of total saponins of astragalus(TSA) on ferroportin 1 in rats with anemia of ahronic disease(ACD) induced by complete Freund’s adjuvant.Method:Complete Freund’s adjuvant was intradermally injected into foot pad of rats to duplicate ACD model.Then TSA(different concentration,respectively 50,16,5 mg · kg-1) was given for 7 days by peritoneum.Erythropoietin was used as the positive control.Interleukin-6(IL-6)in serum was detected with(Enzyme linked immunoassay,ELISA) and the Fpn1 mRNA(in spleen,liver and duodenum) and Hamp mRNA(in liver) were detected with Quantitative real-time Polymerase Chain Reaction(qPCR).Result:IL-6 in serum,Fpn1 mRNA in liver and duodenum,Hamp mRNA in liver were increased obviously(P<0.05),but there wasn’t any change in Fpn1 mRNA in spleen.Compared with model group,IL-6 were decreased significantly both by high and middle dose(P<0.05),but indistinctively by low dose.Fpn1 mRNA in liver and duodenum,Hamp mRNA in liver were decreased significantly and Fpn1 mRNA in spleen was increased in all TSA groups(P<0.05).Otherwise,the effect of TSA on above-mentioned indexes was prior to positive drug(P<0.05).Conclusion:There was obvious increasing of Fpn1 in liver and duodenum in ACD rats,but almost no change in spleen.Fpn1 decreasing corresponding to hepcidin increasing were not found in this experiment.TSA was found to have different effect to Fpn in different tissue.