| 绞股蓝总皂苷对人肝癌细胞Bel-7402增殖与凋亡的影响 |
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| 引用本文： | 李晓龙,吴育朗,林子群,莫淼,梁雅杏,潘越,刘诗良.绞股蓝总皂苷对人肝癌细胞Bel-7402增殖与凋亡的影响[J].中国实验方剂学杂志,2012,18(19):238-241. |
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| 作者姓名： | 李晓龙 吴育朗 林子群 莫淼 梁雅杏 潘越 刘诗良 |
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| 作者单位： | 广西医科大学,南宁,530021 |
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| 基金项目： | 广西医科大学青年科学基金项目(02602211010);实验室开放课题(02608211024) |
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| 摘 要： | 目的:探讨绞股蓝总皂苷(gypenosides,Gyp)对人肝癌细胞Bel-7402增殖与凋亡的影响.方法:采用MTT法检测Gyp对体外培养的人肝癌细胞Bel-7402的增殖抑制作用;单细胞凝胶电泳法检测Gyp对人肝癌细胞Bel-7402 DNA损伤的影响;RT-PCR技术从分子水平上检测Gyp对人肝癌细胞Bel-7402的诱导凋亡作用机制.结果:100 ～400 μg· mL-1的Gyp对人肝癌细胞Bel-7402增殖生长有抑制作用,Gyp(182 μg·mL-1)作用4,8,12,16,24 h后,单细胞凝胶电泳观测到DNA损伤,12h时DNA损伤最大,达到(81.15±14.23)％,且有时间依赖性;Gyp组细胞caspase-8大量表达,对照组细胞无表达.结论:Gyp能抑制人肝癌细胞Bel-7402增殖且促进其凋亡,其作用机制与DNA损伤以及caspase-8的大量表达有关.
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| 关 键 词： | 绞股蓝总皂苷 人肝癌细胞Bel-7402 增殖与凋亡 |
| 收稿时间： | 2012/5/23 0:00:00 |
Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells |
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| LI Xiao-long,WU Yu-lang,LIN Zi-qun,MO Miao,LIANG Ya-xing,PAN Yue and LIU Shi-liang.Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells[J].China Journal of Experimental Traditional Medical Formulae,2012,18(19):238-241. |
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| Authors: | LI Xiao-long WU Yu-lang LIN Zi-qun MO Miao LIANG Ya-xing PAN Yue and LIU Shi-liang |
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| Institution: | Guangxi Medical University, Nanning 530021, China;Guangxi Medical University, Nanning 530021, China;Guangxi Medical University, Nanning 530021, China;Guangxi Medical University, Nanning 530021, China;Guangxi Medical University, Nanning 530021, China;Guangxi Medical University, Nanning 530021, China;Guangxi Medical University, Nanning 530021, China |
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| Abstract: | Objective:To investigate the suppressive effect of gypenosides(Gyp) on human hepatocellular carcinoma Bel-7402 cells.Method:The growth inhibitation of human hepatocellular carcinoma Bel-7402 cells was detected by MTT assay.The DNA damage of human hepatocellular carcinoma Bel-7402 cells was detected by single cell gel electrophoresis;RT-PCR was utilized to investigate the apoptosis of human hepatocellular carcinoma Bel-7402 cells and its relationship with caspase-8 from molecule level.Result: Gyp(100-400 μg · mL-1) inhibied the growth of human hepatocellular carcinoma Bel-7402 cells.DNA damage was detected after Gyp(182 μg · mL-1) treated for 4,8,12,16,24 h.The change reached maximum at 12 h(81.15±14.23)% and with time-dependent.caspase-8 expressed strongly in Gyp group and nothing in control.Conclusion:Gyp has effects on cell viability,it could induce human hepatocellular carcinoma Bel-7402 cells apoptosis,DNA damage and caspase-8 expression. |
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| Keywords: | gypenosides human hepatocellular carcinoma Bel-7402 cells proliferation and apoptosis |
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