外源性硫化氢对双侧卵巢摘除骨质疏松模型大鼠骨代谢的影响

背景:外源性硫化氢(H2S)对成骨细胞的形成和分化有重要的影响,成骨细胞是骨质疏松疾病发生发展的重要功能细胞,而外源性H2S对骨质疏松水平及相关激素的影响尚未见研究.目的:探讨内源性H2S浓度与骨质疏松的相关性,以及补充外源性H2S对骨代谢临床随访常用生化、激素指标的影响.方法:纳入60只大鼠,随机分为3组(n=20)...

Effect of exogenous hydrogen sulfide on bone metabolism in an ovariectomized osteoporotic rat model

BACKGROUND:Exogenous hydrogen sulfide has an important effect on the formation and differentiation of osteoblasts,which are important functional cells in the development of osteoporotic disease.The effect of exogenous hydrogen sulfide on osteoporosis levels and related hormones has not been studied.OBJECTIVE:To investigate the correlation between endogenous hydrogen sulfide concentration and osteoporosis,and the effect of exogenous hydrogen sulfide supplementation on the biochemical and hormonal parameters commonly used in the clinical follow-up of bone metabolism.METHODS:Sixty rats were randomly divided into sham-operated group,model group,and exogenous hydrogen sulfide group(n=20 per group).A classical osteoporosis model was established by removing both ovaries of rats in the latter two groups.No ovariectomy was conducted in the sham-operated group.Rats in the sham-operated and model groups were intraperitoneally given 1 mg/kg normal saline,while those in the exogenous hydrogen sulfide group intraperitoneally injected 100μg/kg GYY4137,a slow-released agent of exogenous hydrogen sulfide.Treatments in each group were conducted twice a day,for 12 weeks.Plasma hydrogen sulfide concentration,blood calcium and phosphorus concentrations,alkaline phosphatase activity,osteocalcin,calcitonin,parathyroid hormone,and leptin laboratory index data of each group were analyzed by quantitative comparison.RESULTS AND CONCLUSION:Bone mineral density of the L5,plasma hydrogen sulfide,calcium,phosphorus,alkaline phosphatase,calcitonin concentrations were significantly lower,and osteocalcin,parathyroid hormone,and leptin levels were significantly higher in the model group compared with the shamoperated group at weeks 8 and 12(P<0.05).There were no significant differences between the sham-operated and exogenous hydrogen sulfide groups at weeks 8 and 12 in terms of L5 bone mineral density,plasma hydrogen sulfide,phosphorus,osteocalcin,calcitonin,parathyroid hormone and leptin levels(P>0.05).Whereas blood calcium concentration and alkaline phosphatase activity were still decreased in the exogenous hydrogen sulfide group compared with the sham-operated group(P<0.05).Compared with the model group,plasma hydrogen sulfide,blood calcium and phosphorus concentrations were elevated and leptin level was decreased in the exogenous hydrogen sulfide group(P<0.05),while the differences in L5 bone mineral density,alkaline phosphatase activity,osteocalcin,calcitonin and parathyroid hormone levels at weeks 8 and 12 were not significant between the two groups(P>0.05).Therefore,in the ovariectomized osteoporotic rat model,endogenous hydrogen sulfide concentration was negatively correlated with the severity of osteoporosis,and exogenous supplementation to increase plasma hydrogen sulfide concentration could improve or reverse plasma calcium,phosphorus and some hormone indexes related to bone metabolism.To conclude,plasma hydrogen sulfide concentration and osteoporosis are correlated,and exogenous hydrogen sulfide supplementation has a potential therapeutic effect on osteoporosis which is caused by estrogen reduction.