白芍总苷对骨关节炎软骨细胞炎症及退变的影响

背景:软骨退变是骨关节炎的病理表现之一,在骨关节炎发病进展中起着重要作用.白芍总苷是白芍的有效成分,有很强的抗炎、止痛、免疫调节作用,然而其对骨关节炎软骨细胞炎症及退变的影响仍不明确.目的:观察白芍总苷对骨关节炎软骨细胞中白细胞介素1β、肿瘤坏死因子α及基质金属蛋白酶13水平的影响.方法:应用胶原酶消化法从膝关节置换手...

Effect of total glucosides of paeony on inflammation and degeneration of chondrocytes in osteoarthritis

BACKGROUND:Cartilage degeneration is one of the most characteristic pathological manifestations of osteoarthritis,which plays an important role in the pathogenesis of osteoarthritis.The total glucosides of paeony(TGP)is the effective components of white paeony root,which has strong anti-inflammatory,analgesic and immunomodulatory effects.However,its effect on inflammation and degeneration of cartilage is still unclear.OBJECTIVE:To observe the effects of TGP on interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and matrix metalloproteinase-13(MMP-13)in osteoarthritic chondrocytes in osteoarthritis.METHODS:Chondrocytes were extracted from the cartilage tissue discarded by knee replacement surgery by collagenase digestion.The effects of different concentrations on the proliferation of chondrocytes were detected by cell counting kit-8 method.The cultured cells were further divided into five groups:blank group,lipopolysaccharide(10 mg/L)intervention group,low concentration TGP group(10μmol/L TGP+10 mg/L lipopolysaccharide),medium concentration TGP group(20μmol/L TGP+10 mg/L lipopolysaccharide)and high concentration TGP group(50μmol/L TGP+10 mg/L lipopolysaccharide).Except for the normal group,the other four groups were induced with lipopolysaccharide to induce inflammation.Real-time quantitative PCR and western blot assay were used to detect the expression of IL-1β,TNF-αand MMP-13 in each group at 24 hours after intervention.RESULTS AND CONCLUSION:TGP of 10,20 and 50μmol/L significantly stimulated the proliferation of chondrocytes,and excessive concentration of TGP significantly inhibited the proliferation.Chondrocytes with lipopolysaccharide induction significantly increased the expression of IL-1β,TNF-αand MMP-13(P<0.01),while TGP could significantly reduce IL-1β,TNF-αand MMP-13 expression in lipopolysaccharide-induced chondrocytes in a concentration-dependent manner(P<0.05,P<0.01).To conclude,TGP has an obvious anti-inflammatory effect and delays extracellular matrix loss in chondrocytes.