自体、同种异体纯化富血小板血浆治疗兔膝骨关节炎的差异

背景:活化后的纯化富血小板血浆可促进软骨细胞的增殖,调控关节内炎症反应,有效治疗膝骨关节炎.目的:对比自体、同种异体纯化富血小板血浆修复膝骨关节炎的效果.方法:取32只3月龄新西兰大耳兔,采用随机数字表法分4组,每组8只:正常组不作任何处理;模型组、自体纯化富血小板血浆组、异体纯化富血小板血浆组通过关节内注射木瓜蛋白酶...

The difference between autologous and allogeneic purified platelet-rich plasma in the treatment of rabbit knee osteoarthritis

BACKGROUND:Purified platelet-rich plasma after activation can promote the proliferation of chondrocytes,regulate inflammation in the joints,and effectively treat knee osteoarthritis.OBJECTIVE:To compare the effects of autologous and allogeneic purified platelet-rich plasma in repairing knee osteoarthritis.METHODS:Thirty-two 3-month-old New Zealand big-eared rabbits were divided into four groups using a random number table method,with eight rabbits in each group:the normal group was treated without any treatment;in the model,autologous,and allogeneic groups,a knee osteoarthritis model was prepared by intra-articular injection of a mixed solution of papain and L-cysteine.After 2 weeks of modeling,the autologous group and allogeneic group were injected intra-articularly with autologous and allogeneic purified platelet-rich plasma,respectively.Platelet-rich plasma was injected once every 2 weeks for a total of three injections.Two weeks after the last injection,gross observation,histological observation and immunohistochemical observation of the articular cartilage were performed.The experiment was approved by the Laboratory Animal Management Committee of Southwest Medical University.RESULTS AND CONCLUSION:General observation:The surface of the articular cartilage of the model group was rough,cartilage defects,erosions,ulcers,joint osteophytes were formed,and there was a large amount of joint cavity effusion.Articular cartilage damage and osteophytes formation in the autologous group were lighter than those in the model group.Compared with the model group,the articular cartilage injury and osteophyte formation in the allogeneic group were not significantly improved.Histological observation:Compared with the model group,the cartilage structure,matrix staining,cells,and tide lines of the autologous group were significantly improved(P<0.05);the cartilage structure of the allogeneic group was improved(P<0.05),but there was no obvious improvement in cell arrangement,matrix staining,and tide line.Immunohistochemical observation:The expression of bone morphogenetic protein 2 and Sox9 in the autologous group and the allogeneic group was higher than that of the model group(P<0.05),and the expression of bone morphogenetic protein 2 in the autologous group was higher than that of the allogeneic group(P<0.05).To conclude,both autologous and allogeneic purified platelet-rich plasma can improve the proliferation of knee osteoarthritis chondrocytes and promote chondrocyte repair to a certain extent,but the therapeutic effect of autologous purified platelet-rich plasma is more obvious.