人乳腺癌外周血中hMAM mRNA表达及临床意义

目的：研究人乳腺癌外周血珠蛋白（hMAM）mRNA表达及临床意义。方法：应用nested-RT-PCR技术检测50例乳腺癌外周血hMAM mRNA的表达和化疗前后外周血hMAM mRNA的变化,并取20例乳腺纤维腺瘤、10例健康志愿者作阴性对照。结果：50例乳腺癌外周血hMAM mRNA的阳性表达率为34.0%;20例乳腺纤维腺瘤及10例健康志愿者外周血中均未检出hMAM mRNA的表达。hMAM mRNA的阳性与淋巴结转移状况、肿瘤TNM分期之间差异有统计学意义;50例乳腺癌化疗前检测17例hMAM mRNA阳性,化疗2～3周期后13例hMAM mRNA转为阴性,转阴率76.5%（13/17）,化疗前后差异非常显著（P=0.001）。结论：乳腺癌外周血中hMAM mRNA表达可以反映术后化疗对乳腺癌血液微转移的影响,有望成为乳腺癌血道微转移标志物和一个重要的预后指标。

The clinical significance of hMAM mRNA expression in peripheral blood of patients with breast cancer

Objective:To study the clinical significance of hMAM mRNA in peripheral blood of patients with breast cancer.Methods:Expression of hMAM mRNA was detected in peripheral blood from patients with breast cancer,breast benign lesions,healthy volunteers by nested-RT-PCR method,the possible correlation of hMAM mRNA expression with clinico-pathological parameters and related molecular markers such as p53,PCNA and HER-2 were analyzed.The hMAM mRNA changed in peripheral blood after chemotherapy was observed.Results:The positive rates of hMAM mRNA was 34.0% in peripheral blood of breast cancer,hMAM mRNA was not expressed in peripheral blood from other cancers,breast benign lesions or healthy volunteers.The expression of hMAM mRNA in peripheral blood was correlated with lymph nodes status and TNM stage.After 2-3 cycle chemotherapy,13 cases in 17cases of positive hMAM mRNA turned to be negative,there was statistical difference between pre-chemotherapy and post-chemotherapy.Conclusion:hMAM mRNA is specific expression in breast cancer,and the positive expression of hMAM mRNA in peripheral blood might predict hematogenous metastatic spreading of tumor cells,and hMAM mRNA may be potential biological marker for detecting breast cancer micrometastasis.The positive expression of hMAM mRNA in peripheral blood might predict hematogenous metastatic spreading of tumor cells,and hMAM mRNA may be potential biological marker for detecting breast cancer micrometastasis.