仙灵骨葆胶囊对骨质疏松性骨折大鼠骨生长因子及骨折愈合的影响

目的 探讨仙灵骨葆胶囊对骨质疏松性骨折大鼠骨生长因子BMP-2、IGF-1表达及骨折愈合的影响。方法 48只雌性SD大鼠随机分为：假手术组、模型组、雌二醇组、仙灵骨葆组,12只/组,采用“双侧卵巢切除术+右侧股骨干骨折髓内固定术”构建骨质疏松性骨折大鼠模型,评估骨折愈合情况,检测股骨骨痂BMD、股骨骨生物力学指标和血清骨代谢相关指标,检测骨痂BMP-2、IGF-1蛋白表达。结果 模型组较假手术组骨折愈合评分、股骨痂BMD、股骨骨生物力学指标（最大载荷、最大应力、最大位移）、骨痂BMP-2和IGF-I阳性表达均显著降低（P<0.05）,雌二醇组、仙灵骨葆组较模型组骨折愈合评分、股骨痂BMD、股骨骨生物力学指标、骨痂BMP-2和IGF-I阳性表达均显著升高（P<0.05）,均以仙灵骨葆组最高。模型组较假手术组血清骨代谢指标（BGP、PICP、TRACP-5b）均显著升高（P<0.05）,雌二醇组、仙灵骨葆组较模型组血清骨代谢指标均显著降低（P<0.05）,以仙灵骨葆组最低。结论 仙灵骨葆胶囊可能通过介导提高骨质疏松性骨折大鼠骨生长因子BMP-2和IGF-1表达,改善骨代谢,加速骨痂形成,增加骨密度,提高骨生物力学,促进骨折愈合。

Effect of Xianling Gubao Capsule on bone growth factor and fracture healing in rats with osteoporotic fracture

Objective To study and explores the effect of Xianling Gubao Capsule on the expression of bone growth factor BMP-2 and IGF-1 and fracture healing in rats with osteoporotic fracture. Methods 48 female SD rats were randomly divided into: Sham operation group, Model group, Estradiol group, Xianling Gubao group, 12 rats/group, "Bilateral oophorectomy + intramedullary fixation of right femoral shaft fracture" was used to construct a rat model of osteoporotic fracture, Assess fracture healing, detect femoral callus BMD, femoral bone biomechanics indicators and serum bone metabolism related indicators, and detect the protein expression of callus BMP-2 and IGF-1. Detection of femoral callus BMD, femoral bone biomechanics indexes and serum bone metabolism related indexes in each group, Immunohistochemical detection of BMP-2 and IGF-1 protein expression in callus of each group. Results Compared with the sham operation group, fracture healing score, femoral callus BMD, femoral bone biomechanics indexes (maximum load, maximum stress, maximum displacement), callus BMP-2 and IGF-I positive expression were significantly lower than the model group (P<0.05), Compared with the model group, the estradiol group and the Xianling Gubao group had significantly higher fracture healing scores, femoral callus BMD, femoral bone biomechanics indexes, callus BMP-2 and IGF-I positive expressions (P<0.05), Xianling Gubao group is the highest. The serum bone metabolism indexes (BGP, PICP, TRACP-5b) of the model group were significantly higher than the sham operation group (P<0.05), The serum bone metabolism indexes of the estradiol group and the Xianling Gubao group were significantly lower than the model group (P<0.05), and the Xianling Gubao group was the lowest. Conclusion Xianling Gubao Capsule may mediate to increase the expression of bone growth factor BMP-2 and IGF-1 in rats with osteoporotic fractures, improve bone metabolism, accelerate callus formation, increase bone density, improve bone biomechanics, and promote fracture healing.