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| 顺铂降低铜离子转运蛋白1表达诱导人食管鳞癌细胞耐药 | |
| 引用本文： | 余乐,陈明辉,古春萍,李亦蕾,曹之宪,刘叔文. 顺铂降低铜离子转运蛋白1表达诱导人食管鳞癌细胞耐药[J]. 中国药理学通报, 2011, 27(7): 911-914. DOI: 10.3969/j.issn.1001-1978.2011.07.007 |
| 作者姓名： | 余乐 陈明辉 古春萍 李亦蕾 曹之宪 刘叔文 |
| 作者单位： | 1. 南方医科大学,药学院,广东,广州,510515 2. 南方医科大学,附属南方医院药学部,广东,广州,510515 3. 香港中文大学生物医学学院,香港 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的诱导对顺铂耐药的人食管鳞癌细胞株,并进一步研究细胞耐药性产生的机制。方法逐渐增加顺铂浓度处理人食管鳞癌细胞HKESC-1,诱导顺铂耐药细胞株HKESC-1/cis。MTT法观察顺铂的细胞毒作用以及细胞的生长曲线。电感耦合等离子体质谱检测细胞内顺铂的蓄积和Pt-DNA加合物的形成。Western blot检测铜离子转运蛋白(copper transporter 1,CTR1)的表达。结果顺铂耐药细胞株HKESC-1/cis较其亲代细胞HKESC-1对顺铂引起的细胞毒作用敏感性下降,耐药指数为2.9。顺铂耐药细胞株HKESC-1/cis与其亲代细胞HKESC-1的生长速度相似。然而,HKESC-1/cis细胞内顺铂蓄积和Pt-DNA加合物的形成较HKESC-1细胞减少,并且CTR1蛋白表达水平降低。结论顺铂通过降低细胞膜CTR1蛋白的表达,抑制顺铂进入细胞,导致细胞耐药性的产生。 |
| 关 键 词： | 食管鳞癌 顺铂 耐药性 铜转运蛋白1 Pt-DNA加合物 细胞毒作用 |
Cisplatin induces drug resistance in human esophageal squamous carcinoma cells by reducing CTR1 expression |
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| YU Le,CHEN Ming-hui,GU Chun-ping,LI Yi-lei,CAO Zhi-Xian,LIU Shu-wen. Cisplatin induces drug resistance in human esophageal squamous carcinoma cells by reducing CTR1 expression[J]. Chinese Pharmacological Bulletin, 2011, 27(7): 911-914. DOI: 10.3969/j.issn.1001-1978.2011.07.007 | |
| Authors: | YU Le CHEN Ming-hui GU Chun-ping LI Yi-lei CAO Zhi-Xian LIU Shu-wen |
| Affiliation: | YU Le1,CHEN Ming-hui1,GU Chun-ping1,LI Yi-lei2,CAO Zhi-Xian3,LIU Shu-wen1 (1.School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,China,2.Dept of Pharmacy,Nanfang Hospital,3.School of Biomedical Sciences,the Chinese University of Hong Kong,Hong Kong,China) |
| Abstract: | Aim To induce cisplatin-resistant esophageal squamous carcinoma cell line and investigate the mechanisms by which the cancer cells develop drug resistance.Methods The cisplatin-resistant subline,designated HKESC-1/cis,was originated by growing parental HKESC-1 cells with gradually increasing doses of cisplatin.The cytotoxicity of cisplatin and cell growth curves were determined by MTT assay.Whole-cell cisplatin accumulation and Pt-DNA adduct formation were measured by Inductively Coupled Plasma Mass Spectro... |
| Keywords: | esophageal squamous cell carcinoma cisplatin resistance CTR1 Pt-DNA adduct cytotoxicity |
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