| HPLC法测定栽培亚大黄中3种成分的含量 |
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| 引用本文： | 耿家玲,沈勇,康绍建.HPLC法测定栽培亚大黄中3种成分的含量[J].中国药师,2011,14(5):666-668. |
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| 作者姓名： | 耿家玲 沈勇 康绍建 |
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| 作者单位： | 1. 云南省食品药品检验所,昆明,650011
2. 昆明植物研究所 |
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| 摘 要： | 目的:建立HPLC法分离和测定亚大黄药材中虎杖苷、白藜芦醇、甲基虎杖苷的含量。方法:色谱柱:C18柱（250 mm×4.6 mm,5μm）;流动相:乙腈-甲醇-0.1%磷酸（16:8:76）梯度洗脱,检测波长:306 nm。结果:虎杖苷在0.009 1～0.454 0μg;白藜芦醇在0.000 6～0.030 4μg;甲基虎杖苷在0.047 4～2.370 0μg范围内与峰面积呈良好的线性关系,平均回收率分别为98.82%（RSD=1.2%）,98.33%（RSD=2.1%）,99.40%（RSD=1.5%）。结论:本方法可作为亚大黄药材质量控制手段之一。
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| 关 键 词： | 亚大黄 虎杖苷 白藜芦醇 甲基虎杖苷 HPLC法 |
Determination of three Compositions in Cultivated Rheum Ihasaense by HPLC |
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| Geng Jialing,Shen Yong,Kang Shaojian.Determination of three Compositions in Cultivated Rheum Ihasaense by HPLC[J].China Pharmacist,2011,14(5):666-668. |
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| Authors: | Geng Jialing Shen Yong Kang Shaojian |
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| Institution: | Geng Jialing~1,Shen Yong~2,Kang Shaojian~1(1.Yunnan Institute for Food and Drug Control,Kunming 650011,China,2.Kunming Institute for Plant Study) |
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| Abstract: | Objective： To establish a method for the determination of polydatin, resveratrol and deoxyrhapontin in Rheum lhasaense. Method： On a C18 column （250 mm ×4. 6 mm ,5 μm）, acetonitrile-methanol-0. 1% phosphoric acid （16： 8： 76）was used as the mobile phase with gradient elution and UV detection wavelength was set at 306 nm. Result： There was a good linear relationship within the range of 0. 009 1-0. 454 0 9μg,0. 000 6-0. 030 4μg and 0. 047 4-2. 370 0μg for polydatin,resveratrol and deoxyrhapontinm, respectively. The average recovery for polydatin was 98. 82% , 98.33% for resveratrol and 99. 40% for deoxyrhapontin. Conclusion： The method can be applied in the determination of polydatin, resveratrol and deoxyrhapontin in Rheum Ihasaense. |
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| Keywords: | Rheum Ihasaense Polydatin Resveratro Deoxyrhapontin HPLC |
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