| 多巴胺D3受体对大鼠肾脏近曲小管上皮细胞多巴胺D4受体表达和功能的影响 |
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| 引用本文： | 陈新建,杨剑,任红梅,陈彩宇,何多芬,王旭开,王红勇,杨成明,周林,曾春雨. 多巴胺D3受体对大鼠肾脏近曲小管上皮细胞多巴胺D4受体表达和功能的影响[J]. 中国循环杂志, 2011, 26(2). DOI: 10.3969/j.issn.1000-3614.2011.02.019 |
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| 作者姓名： | 陈新建 杨剑 任红梅 陈彩宇 何多芬 王旭开 王红勇 杨成明 周林 曾春雨 |
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| 作者单位： | 中国人民解放军第三军医大学第三附属医院,心内科,重庆市,400042 |
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| 基金项目： | 国家杰出青年科学基金，重庆市杰出青年科学基金，国家自然基金资助项目 |
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| 摘 要： | 目的:观察多巴胺D3受体(D3受体)对大鼠肾脏近曲小管(RPT)上皮细胞多巴胺D4受体(D4受体)表达和功能的影响.方法:以Wistar-Kyoto(WKY)大鼠RPT上皮细胞为研究对象,利用免疫印迹法观察D3受体激动剂PD128907刺激D3受体后D4受体表达的变化;采用哇巴因法测定Na+-K+-ATP酶(ATP为三磷酸腺苷)活性,观察D3受体激动剂PDl28907(10-7mol/L,24 h)预先作用后,D4受体激动剂PD168077(10-7 mol/L,15 min)对Na+-K+-ATP酶活性的影响.同时,探讨D3受体激动剂PDl28907影响D4受体表达的作用机制.结果:D3受体激动剂PDl28907刺激可明显增强RPT上皮细胞中的D4受体蛋白表达,该作用呈时间与浓度依赖性关系.D3受体激动剂PDl28907对D4受体蛋白表达的增强作用可被D3受体特异性抑制剂U99194A(10-6 mol/L)所阻断.磷脂酶C抑制剂U73122存在的情况下,D3受体激动剂PD128907刺激D3受体后对D4受体的促进表达效应受到显著抑制.与单用D4受体激动剂PD168077相比,在PDl28907(10-7 mol/L,24 h)预先刺激的情况下,D4受体激动剂PD168077对Na+-K+-ATP酶活性的抑制作用有所增强.结论:D3受体对大鼠RPT上皮细胞上D4受体表达和功能具有增强作用,此作用可能通过磷脂酶C信号途径发挥影响.
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| 关 键 词： | 多巴胺D3受体 多巴胺D4受体 肾脏近曲小管上皮细胞 |
Effect of D3 Dopamine Receptor on D4 Dopamine Receptor Protein Expression and its Biological Function in Rats' Renal Proximal Tubular Cells |
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| CHEN Xin-jian,YANG Jian,REN Hong-mei,CHEN Cai-yu,HE Duo-fen,WANG Xu-kai,WANG Hong-yong,YANG Cheng-ming,ZHOU Lin,ZENG Chun-yu. Effect of D3 Dopamine Receptor on D4 Dopamine Receptor Protein Expression and its Biological Function in Rats' Renal Proximal Tubular Cells[J]. Chinese Circulation Journal, 2011, 26(2). DOI: 10.3969/j.issn.1000-3614.2011.02.019 |
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| Authors: | CHEN Xin-jian YANG Jian REN Hong-mei CHEN Cai-yu HE Duo-fen WANG Xu-kai WANG Hong-yong YANG Cheng-ming ZHOU Lin ZENG Chun-yu |
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| Affiliation: | CHEN Xin-jian,YANG Jian,REN Hong-mei,CHEN Cai-yu,HE Duo-fen,WANG Xu-kai,WANG Hong-yong,YANG Cheng-ming,ZHOU Lin,ZENG Chun-yu.Department of Cardiology,DaPing Hospital,The Third Military Medical University,Chongqing(400042),China |
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| Abstract: | Objective:To investigate the effect of D3 dopamine receptor on D4 dopamine receptor protein expression and function in rats' renal proximal tubular(RPT)cells.Methods:Immortalized RPT cells from Wistar-Kyoto rats were stimulated by D3 dopamine receptor agonist PD128907.The protein expression of D4 dopamine receptor was examined by immunoblotting and its biological function was determined by Na+-K+-ATPase activity.Results:D3 dopamine receptor agonist PD128907 increased D4 dopamine receptor protein expression ... |
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| Keywords: | D3 dopamine receptor D4 dopamine receptor Renal proximal tubular cells |
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