| 大孔吸附树脂对地榆中总鞣质的纯化 |
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| 引用本文： | 周本宏,张琛霞,沈恒,邱振鹏.大孔吸附树脂对地榆中总鞣质的纯化[J].中国药师,2011,14(5):685-688. |
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| 作者姓名： | 周本宏 张琛霞 沈恒 邱振鹏 |
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| 作者单位： | 1. 武汉大学人民医院,武汉,430060;武汉大学药学院
2. 武汉大学药学院 |
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| 摘 要： | 目的：筛选最佳大孔树脂纯化地榆中总鞣质，研究其最佳工艺条件及参数。方法：以鞣质的吸附量、吸附率和解吸率为考察指标，对8种大孔吸附树脂并进行评价。结果：HPD-400型树脂具有较好的吸附分离性能，最佳工艺条件为：上样液浓度4．54mg·ml^-1，吸附流速为4BV·h^-1，上柱量为4BV，以70％的乙醇洗脱，用量为3BV。通过大孔树脂纯化后，终产品中鞣质的纯度为68．52％。结论：HPD-400型树脂可用于地榆中鞣质的富集纯化。
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| 关 键 词： | 大孔吸附树脂 鞣质 纯化 |
Separation and Purification of Tannins from Sanguisorba by Macroporous Adsorbent Resin |
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| Zhou Benhong,Zhang Chenxia,Shen Heng,Qiu Zhenpeng.Separation and Purification of Tannins from Sanguisorba by Macroporous Adsorbent Resin[J].China Pharmacist,2011,14(5):685-688. |
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| Authors: | Zhou Benhong Zhang Chenxia Shen Heng Qiu Zhenpeng |
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| Institution: | Zhou Benhong~(1,2),Zhang Chenxia~2,Shen Heng~2,Qiu Zhenpeng~2(1.Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China,2.College of Pharmacy Wuhan University) |
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| Abstract: | Objective ： To optimize the macroporous resin for the purification of tannins from Sanguisorha , and study the technical parameters. Method： Taking the absorptive capacity , the adsorption ratio and eluting ratio of tannins as standards , eight types of macroporous adsorption resins were investigated and evaluated. Result： HPD-400 was the best one to be chosen , the optimal technological conditions are as follows ：4. 54 mg· ml^- 1 of sample concentration, 4 BV· h ^-1 of sample velocity, 4 BV of sample quantity, 70% ethanol is used as eluant , and eluant 3 BV can completely desorb the tannins adsorbed on the column. The product purity is up to 68.52% after purification. Conclusion： HPD-400 resin can be used to refine the tannins in Sanguisorba. |
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| Keywords: | Macroporous adsorption resin Tannin Purification |
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