| 针刺对膝骨关节炎大鼠软骨基质金属蛋白酶及其抑制剂表达的影响 |
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| 作者姓名： | Bao F Sun H Wu ZH Wang DH Zhang YX |
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| 作者单位： | 1. 中国医学科学院北京协和医学院北京协和医院,中医科,北京,100730
2. 中国医学科学院北京协和医学院北京协和医院,骨科,北京,100730 |
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| 基金项目： | 国家中医药管理局中医药科学技术研究专项课题 |
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| 摘 要： | 目的:探讨针刺治疗骨关节炎(OA)的作用机制.方法:选用SD雌性大鼠40只,采用随机数字表随机分为正常组、模型组、针刺组和药物组,每组10只.单侧后肢跟腱切除建立OA动物模型,针刺组采用电针大鼠"膝前"穴和"后三里"穴,药物组采用膝关节处涂擦扶他林乳剂进行治疗,正常组与模型组不予干预.采用免疫组化技术,观察各组关节软骨中基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)及组织金属蛋白酶抑制剂-1(TIMP-1)表达的特点,并比较各组间的差异.结果:4组组间差异非常显著,模型组、针刺组和药物组的MMP-1、MMP-3和TIMP-1表达均较正常组显著上调(均P<0.01);针刺组和药物组与模型组比较,均下调MMP-1和MMP-3,上调TIMP-1,差异均有统计学意义(均P<0.01);但针刺组MMP-1和MMP-3的表达显著低于药物组(均P<0.01),而TIMP-1的表达则显著高于药物组(P<0.01)o结论:针刺对OA软骨细胞MMP-1、MMP-3、TIMP-1的表达具有影响作用,下调MMP-1、MMP-3,上调TIMP-1,作用优于扶他林乳剂,说明针刺对OA软骨具有一定的保护作用.
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| 关 键 词： | 骨关节炎 膝 电针 基质金属蛋白酶 组织金属蛋白酶抑制剂 |
Effect of acupuncture on expression of matrix metalloproteinase and tissue inhibitor in cartilage of rats with knee osteoarthritis |
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| Bao F,Sun H,Wu ZH,Wang DH,Zhang YX.Effect of acupuncture on expression of matrix metalloproteinase and tissue inhibitor in cartilage of rats with knee osteoarthritis[J].Chinese Acupuncture & Moxibustion,2011,31(3):241-246. |
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| Authors: | Bao Fei Sun Hua Wu Zhi-Hong Wang Dao-Hai Zhang Yun-Xiang |
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| Institution: | BAO Fei1,SUN Hua1,WU Zhi-hong2,WANG Dao-hai1,ZHANG Yun-xiang1(1.Department of TCM,2.Department of Orthopaedics,Peking Union Medical College Hospital,Chinese Union Medical College and Chinese Academy of Medical Sciences,Beijing 100730,China) |
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| Abstract: | Objective To explore the curative mechanism of acupuncture treatment on osteoarthritis(OA).Methods Forty cases of female SD rats were randomly divided into a normal group,a model group,an acupuncture group and a medication group,10 cases in each gruop.OA animal model was established by using the method of heel tendon resection for unilateral hind limb.The acupuncture group was treated with electroacupuncture at Xiqian(ST 35) and Housanli(ST 36),and the medication group with inunction of Diclofenac cream,and... |
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| Keywords: | Osteoarthritis Knee Electroacupuncture MMPs TIMPs |
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