灵芝孢子油及灵芝提取物孢子油对乳腺癌血管生成调节因子的作用

目的探讨灵芝孢子油及灵芝提取物孢子油对人源高恶性乳腺癌血管生成调节因子的作用。方法不同剂量的灵芝孢子油、灵芝提取物孢子油分别刺激体外培养的人源高恶性乳腺癌细胞系MDA-MB-231细胞及MDA-MB-231细胞制作的荷廇小鼠,Western blotting检测表皮生长因子受体(EGFR)的蛋白表达变化,Real-time PCR检测肿瘤血管生成相关因子EGFR、血管内皮生长因子(VEGF)、基质金属蛋白酶(MMPs)、血小板反应素-1(TSP-1)、血小板衍生因子(PDGF)、成纤维细胞生长因子(FGF)转录水平的基因表达变化。结果灵芝孢子油、灵芝提取物孢子油均可在体内和体外抑制人源高恶性乳腺癌细胞MDA-MB-231的EGFR蛋白表达,且有剂量依赖性;均可在体内和体外抑制VEGF RNA表达,增强血管生成抑制因子TSP-1 RNA表达;灵芝提取物孢子油比灵芝孢子油的作用更为显著。结论灵芝孢子油、灵芝提取物孢子油均有抑制肿瘤血管生成因子表达和促进血管生成抑制因子表达的作用,其中灵芝提取物孢子油比灵芝孢子油的作用更为显著。

Effects of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil on angiogenesis regulatory factors

Objective To study the role of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil in neovascularization of human high malignant breast cancer. Methods Human high malignant breast cancer cell MDA-MB-231 and tumor-bearing nude mice established with MDA-MB-231 were treated with different doses of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil. Epidermal growth factor receptor(EGFR) expression level was examined by Western blotting and the RNA expression levels of neovascularization related molecules such as EGFR, vascular endothelial growth factor(VEGF), metalloproteinases(MMPs), thrombospondin(TSP-1), platelet derived growth factor(PDGF), fibroblast growth factor(FGF) were detected by Real-time PCR. Results Both ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil inhibited the expression of EGFR in vitroand in vivoin a dose-dependent way. Both compounds induced down-regulation of VEGF and up-regulation of TSP-1 at RNA level. The effect of Ganoderma lucidum extraction spore oil was more significant than that of ganoderma lucidum spore oil. Conclusion Both ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil inhibite the expression of neovascularization related molecules and increase the expression of molecules inhibiting neovascularization, whereas the effect of ganoderma lucidum extraction spore oil is more obvious.