基质金属蛋白酶-1、-2与女性年龄、骨转换生化指标及骨密度的关系

目的 探讨血清基质金属蛋白酶（MMP）-1、MMP-2与女性年龄、骨密度（BMD）及骨转换生化指标之间的关系。方法用ELISA测定591例20-80岁女性志愿者血清MMP-1、MMP-2、血清骨碱性磷酸酶（BAP）、血清骨钙素（OC）和血清Ⅰ型胶原氨基末端肽（NTX）,用DEXA测定腰椎1-4正位总体、股骨颈、Ward三角区、总髋部的BMD。结果（1）MMP-1、MMP-2与年龄呈正相关。（2）绝经后妇女MMP-2水平高于绝经前妇女（P〈0．001）。（3）MMP-2与BMD呈负相关（P〈0．05）,但多元线性回归分析表明MMP-2不是BMD的预测因子。（4）MMP-2与血清BAP、OC、NTX正相关（P〈0．01）。（5）绝经后骨质疏松症患者血清MMP-2水平高于年龄匹配的正常对照组、骨量减少组（P〈0．01）。结论血清MMP-2与骨转换生化指标相关联。血清MMP-2水平升高可能为高骨转换过程（如绝经后骨质疏松症）中的一种伴随表现。

Age-related changes serum levels of MMP-1 and MMP-2 in women: their relationship with bone biochemical markers and bone mineral density

OBJECTIVE: To study the relationship between serum matrix metalloproteinase (MMP)-1, MMP-2 and bone turnover markers, bone mineral density (BMD) in Chinese women aged 20-80 years. METHODS: Serum MMP-1, MMP-2, bone alkaline phosphatase (BAP), osteocalcin (OC) and cross-linked N-telopeptide of collagen type I (NTX) were measured using ELISA. BMD were measured using DEXA in 591 women. RESULTS: (1) MMP-1 and MMP-2 were positively correlated with age (both P < 0.001). (2) Level of MMP-2 in postmenopausal women was significantly higher than that in premenopausal women (P < 0.001). (3) Significant negative relationship was found between MMP-2 and BMD, but multiple linear stepwise regression analysis showed MMP-2 was not the determinant factor of BMD. (4) Positive correlation was found between MMP-2 and BAP, OC, NTX (all P < 0.001). (5) Serum MMP-2 concentrations were significantly higher in postmenopausal osteoporotic women than in age-matched normal controls and osteopenic subjects. CONCLUSION: Circulating MMP-2 and markers of bone turnover are related and they may increase with the increase in bone-turnover. The increase of serum MMP-2 appears possibly as a concomitant event in high bone turnover state such as postmenopausal osteoporosis.