糖尿病周围神经痛患者血清促炎细胞因子水平的相关性研究

目的 检测糖尿病周围神经痛患者血清中巨噬细胞移动抑制因子(MIF)、肿瘤坏死因子(TNF-α),白介素6(IL-6)的水平变化,探讨促炎细胞因子在糖尿病周围神经痛中所发挥的作用.方法 选择无并发症的糖尿病患者(DM)28例、糖尿病周围神经病变患者(DPN)32例、糖尿病周围神经痛患者(DPNP)28例及正常对照人群28名,采用双抗体酶联免疫吸附法检测血清MIF、TNF-α、IL-6的水平.结果 DM及DPN、DPNP组的血清MIF、TNF-α、IL-6水平均高于正常对照组,差异有统计学意义(P<0.001,P<0.05);DPN及DPNP组的血清MIF、TNF-α水平又均高于DM组,差异有统计学意义(P<0.05,P<0.001,P<0.01),而DPN和DPNP组间血清MIF、TNF-α水平比较差异无统计学意义(P>0.05);DM、DPN、DPNP组三者间血清IL-6水平差异无统计学意义(P>0.05).相关性分析结果 显示:血清MIF、TNF-α仅及IL-6水平三者间相互呈正相关(r=0.337,P<0.01;r=0.216,P<0.001;r=0.281,P<0.05).结论血清促炎细胞因子MIF、TNF-α、IL-6水平的升高可能参与了糖尿病及糖尿病周围神经病的发生、发展,但在糖尿病周围神经痛的发生中可能不起主导作用.

Serum levels of pro-inflammatory cytokines in diabetic patients with peripheral neuropathic pain and the correlation among them

Objective To investigate the change of serum levels of the pro-inflammatory cytokines: macrophage migration inhibition factor (MIF), tumor necrosis factor-a( TNF-α), and interleukin 6 (IL-6), in patients with diabetic peripheral neuropathic pain (DPNP) and their role in the pathogenesis of related diseases. Methods Peripheral blood samples were collected from 28 patients with diabetes mellitus (DM) without complications , and gender ratio- and age-matched 32 patients with diabetic peripheral neuropathy ( DPN), 28 patients with DPNP, and 28 normal controls. Dual-antibody ELISA was used to detect the serum MIF, TNF-α, and IL -6 levels. Results The serum MIF, TNF-α, and IL-6 levels of the DM, DPN, and DPNP groups were all significantly higher than those of the control group (P < 0. 001, P < 0. 05). The serum MIF and TNF-α levels of the DPN and DPNP groups were all significantly higher than those of the DM group (P <0. 05 or P < 0. 001 ), while there were not significant differences in the serum MIF and TNF-α levels between the DPN and DPNP groups ( both P > 0. 05 ). There was not significant difference in the serum IL-6 level among the DM, DPN, and DPNP groups as well (all P > 0. 05). The correlation analysis show that the levels of MIF , TNF-α and IL-6 each other were positively correlated (r =0. 337, P <0. 01 ; r=0.216, P<0.001;r=0. 281,P<0. 05). Conclusions The serum pro-inflammatory cytokines MIF, TNF-α, and IL-6 levels play an important role in the pathogenesis of DM and DPN, but may not play an important role in the development of DPNP.