贞清方对2型糖尿病大鼠肾组织基质金属蛋白酶-9和基质金属蛋白酶组织抑制物-1表达的调节作用 |
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引用本文: | 郑龙轶,文秀英,许明望,刘浩,孙立敏,陈斌,熊亮.贞清方对2型糖尿病大鼠肾组织基质金属蛋白酶-9和基质金属蛋白酶组织抑制物-1表达的调节作用[J].中国中西医结合杂志,2006(Z1). |
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作者姓名: | 郑龙轶 文秀英 许明望 刘浩 孙立敏 陈斌 熊亮 |
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作者单位: | 华中科技大学同济医学院附属梨园医院中西医结合科 华中科技大学同济医学院附属梨园医院中西医结合科 武汉 430077 硕士研究生 武汉 430077 导师 |
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基金项目: | 武汉市科技局科技攻关项目(No.20046001047) |
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摘 要: | 目的探讨中药贞清方(含女贞子、地龙等中药)对2型糖尿病大鼠肾脏结构及肾组织基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制物-1(TIMP-1)表达的影响。方法采用高糖高脂饲料喂养加小剂量链脲佐菌素(streptozotocin,STZ)注射的方法建立2型糖尿病模型。将成模大鼠随机分成模型组,贞清方高、低剂量组,依那普利组,并设立正常对照组,治疗8周。用免疫组化法检测MMP-9、TIMP-1以及纤维连结蛋白(FN)在肾脏的表达,光镜观察肾小球和肾小管形态。结果糖尿病模型组较对照组TIMP-1、FN表达增强,MMP-9表达减弱;治疗组TIMP-1、FN表达较模型组减弱,MMP-9表达增强。其中贞清方高剂量组作用最明显。形态学观察显示治疗组肾小球和肾小管病变比模型组减轻。结论贞清方可能通过影响糖尿病大鼠肾脏MMP-9、TIMP-1的表达而发挥其对糖尿病大鼠肾脏结构的保护作用。
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关 键 词: | 贞清方 糖尿病肾病 基质金属蛋白酶-9 基质金属蛋白酶组织抑制物-1 |
Modulative Effect of Zhenqing Recipe on Expressions of Matrix Metalloproteinase-9 and Tissue Inhibitor of Matrix Metalloproteinase-1 in Experimental Type 2 Diabetic Rats |
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Institution: | ZHENG Long-yi WEN Xiu-ying XU Mingwang Department of Integrated Traditional and Western Medicine; Liyuan Hospital Affiliated to Tongji Medical College; Huazhong University of Science and Technology; Wuhan(430077); |
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Abstract: | Objective To explore the effect of Zhenqing Recipe(ZQR)on renal structure and expressions of matrix metalloproteinase-9(MMP-9),tissue inhibitor of matrix metalloproteinase-1(TIMP-1)in experimental type 2 diabetic rats.Methods The rat models of type 2 diabetic were set up by intraperitoneally giving small-dose streptozotocin(STZ)after fed with high carbohydrate and high fat diets for one month.The model rats were ran- domly divided into the model group,the high and low dose ZQR-treated groups,and the enalapr... |
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Keywords: | Zhenqing Recipe diabetic nephropathy matrix metalloproteinase-9 tissue inhibitor of matrix metalloproteinase-1 |
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