排尿梗阻大鼠尿动力学及逼尿肌不稳定动态变化的研究

目的观察大鼠排尿梗阻后膀胱功能的动态变化以及逼尿肌不稳定的发生、发展进程,确定选择不稳定膀胱模型的最佳时期.方法结扎膀胱颈部形成膀胱出口不完全性梗阻,留置膀胱造瘘,清醒状态下进行不同时期膀胱功能尿流动力学检测.结果与对照组相比,所有梗阻组动物膀胱容量、残余尿量均显著增加,排尿压与顺应性增加,出现膀胱白发性收缩.在梗阻的第5、6周,96%的动物发生膀胱不稳定性收缩,并有一相对稳定期,而到第8周,排尿压、顺应性与自发性收缩因膀胱功能严重损害呈下降趋势.结论膀胱出口梗阻后膀胱功能呈进行性损害;梗阻第5、6周的动物是用来研究DI发生机制和病理生理变化的良好模型.

Dynamic analysis of urodynamics and detrusor instability of bladder outlet obstruction in rats

Objective To observe the occurrence of the detrusor instability (DI) in rats after the bladder outlet obstruction and choose the best period for building the model of unstable bladder. Methods Partial obstruction was established by ligation of the bladder neck and the bladder stoma was reserved. Urodynamic indexes in conscious rats at different time points were detected. Results Compared with those in the control group, the bladder volume and the residual urine increased significantly with the enhancement of the voiding pressure and the compliance and the spontaneous contraction activity of the bladder. In the period from 5 to 6 weeks, instability was found in 96% rats, and remained unchanged in a relative phase. However, after 8 weeks, voiding pressure, compliance, and the spontaneous contraction activity of the bladder decreased. Conclusion Bladder functions may be destroyed progressively after bladder outlet obstruction, and the rats between 5 to 6 weeks are the ideal model for the studies of the mechanisms and the pathophysiological changes of DI.