Developmental stage‐specific contribution of LGR5+ cells to basal and luminal epithelial lineages in the postnatal mammary gland |
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| Authors: | Karin E de Visser Metamia Ciampricotti Ewa M Michalak David Wei‐Min Tan Cheei‐Sing Hau Hans Clevers Nick Barker Jos Jonkers |
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| Affiliation: | 1. Division of Immunology, The Netherlands Cancer Institute, , Amsterdam, The Netherlands;2. Division of Molecular Pathology, The Netherlands Cancer Institute, , Amsterdam, The Netherlands;3. Institute of Medical Biology, , Singapore;4. Hubrecht Institute, Developmental Biology and Stem Cell Research, and Utrecht University Medical Centre, , Utrecht, The Netherlands |
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| Abstract: | The leucine‐rich repeat‐containing heterotrimeric guanine nucleotide‐binding protein‐coupled receptor 5 (LGR5) has been identified as a marker of cycling stem cells in several epithelial tissues, including small intestine, colon, stomach and hair follicle. To investigate whether LGR5 also marks mammary epithelial stem cells, we performed in situ lineage‐tracing studies and mammary gland reconstitutions with LGR5‐expressing mammary epithelial cells. Interestingly, the LGR5 progeny population in mammary epithelium switches from the luminal to the myoepithelial compartment during the first 12 days of postnatal development, likely reflecting local changes in Wnt signalling. Together, our findings point to a stage‐specific contribution of LGR5‐expressing cells to luminal and basal epithelial lineages during postnatal mammary gland development. Copyright © 2012 Pathological Society of Great Britain and Ireland. |
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| Keywords: | LGR5 lineage tracing mammary gland postnatal stem cell |
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