转化生长因子-β1在子痫前期患者胎盘组织中的表达研究

目的探讨转化生长因子-β1mRNA（TGF—β1mRNA）在子痫前期发病机制中的作用。方法采用RT—PCR技术检测45例正常晚期妊娠妇女及56例子痫前期患者（其中轻度子痫前期21例，重度子痫前期35例）胎盘组织中TGF－β3mRNA的表达水平。结果轻、重度子痫前期胎盘组织中TGF－β1mRNA表达水平分别为：0．752±0．133、1．0164-0．346均明显高于正常妊娠组0．384±0．098（均P〈0．01），重度子痫前期组高于轻度子痫前期组（P〈0．01）。子痫前期胎盘组织中TGF-β1mRNA表达水平与24h尿蛋白、血压、新生儿体重及胎盘重量均有明显相关性。结论1．子痫前期胎盘组织中TGF-β1mRNA表达水平明显升高，并且与子痫前期病情发展密切相关。2．胎盘组织TGF－β1mRNA的表达水平与子痫前期患者胎儿发育密切相关。

Expression of tumor necrosis factor - α and transforming growth factor - β1 mRNA in placenta in preeclampsia and its significance

Objective： To investigate the placental transforming growth factor - β1 （TGF - β1 ） mRNA in normal pregnancies and patients with preeclampsia and to study their clinical significance. Methods ： RT - PCR was used to measure levels of placental TGF - β1 mRNA in 45 normal late pregnant women and 56 patients with preeclampsia （ 21 mild preeclampsia, 35 severe preeclampsia）. Results ： The levels of TGF - β1 of placenta in mild and severe preeclampsia were 0. 752 ＋ 0. 133, 1. 016 ＋ 0. 346 respectively. There were all obviously significant than normal pregnant group 0. 384 ± 0. 098 （ P 〈 0.01 ）. There was all obviously significant in mild preeclampsia than severe preeclampsia （ P 〈0. 01 ）. TNF - α mRNA and TGF -β1 mRNA levels of placenta in preeclampsia obviously correlated with the levels of SBP, DBP, MAP, proteinuria , the weight of newborn and placenta weight. Conclusion： 1. The results suggests that the levels of TNF -α mRNA and TGF - β1 mRNA in placenta are all gradually increased in preeclampsia, which was not changed in pregnant hypertension. 2. The levels of TNF - α mRNA and TGF - β1 mRNA of placenta obviously correlated with devepement of fetus.