诱导血红素氧合酶-1对小肠移植缺血再灌注损伤的影响

目的：探讨诱导血红素氧合酶-1（HO-1）是否可减轻大鼠小肠移植缺血再灌注损伤及其机制。方法：SD大鼠分成3组：Ⅰ组：血晶素（Hemin）组（n=20）；Ⅱ组：Hemin＋卟啉锌（ZnPP）组（n=20）；Ⅲ组：生理盐水组（n=20）。建立小肠移植缺血再灌注损伤模型。移植后6、12、24、48h分别从各组随机取5只大鼠，切取移植小肠进行HO-1活性测定．普通病理学检查及细胞凋亡检测。结果：术后各时段Ⅰ组HO-1活性均显著强于Ⅱ组及Ⅲ组（P〈0．05），而Ⅱ组和Ⅲ组比较无显著差异（P〉0．05）。各组移植肠缺血再灌注损伤以术后12h最为明显。Ⅰ组表现轻度，而Ⅱ组和Ⅲ组表现中度缺血再灌注损伤。术后各时段Ⅰ组细胞凋亡数少于Ⅱ组和Ⅲ组，差别有统计学意义（P〈0．05），而Ⅱ组和Ⅲ组比较无统计学意义（P〉0．05）。结论：诱导HO-1可通过抑制细胞凋亡而减轻移植肠缺血再灌注损伤。

Influence of induced heme oxygenase-1 on ischemia-reperfusion injury of small bowel transplantation

Objective:To investigate whether induced HO-1 could alleviate the ischemia-reperfusion injury in transplanted small bowel and to investigate the mechanism.Methods:SD rats were divided into three groups:group 1(hemin group,n = 20),group 2(hemin ZnPP group,n = 20)and group 3(saline group,n = 20).Ischemia-reperfulion injury model of small bowel transplantation was established.Five rats of each group were sacrificed 6,12,24 and 48 h after transplantation,then the activity of HO-1 in the tissue of transplanted intestine was detected,histological changes observed and apoptosis detected.Results:The post-operative activity of HO-1 in group 1 was significantly higher than that in group 2 and group 3(P < 0.05),but there was no significant difference in the activity of HO-1 between group 2 and group 3.All rats showed the most serious ischemia-reperfusion injury 12 hours after reperfusion,and the damage was milder in group 1 than that in the other two groups.At the same time,there were less apoptosis cells in group 1 than group 2 and group 3(P < 0.05).Conclusion:HO-1 can alleviate the ischemia-reperfusion injury of transplanted small bowel by reducing apoptosis.