山西籍汉族血管紧张素转换酶基因多态性与高血压遗传的研究

目的　研究山西籍汉族人血管紧张素转换酶 (angiotension converting enzym e,ACE)基因多态性与原发性高血压 (essential hypertension,EH)间的相互关系 ,采用三条引物法进行基因分型检测。方法　对 41个EH核心家系的 12 3例患者以及正常对照组 98名成员 ,用三条引物法进行基因分型检测。结果　 EH组 ,ACE基因型的分布频率为 :DD型 0 .10 ,ID型 0 .40 ,II型 0 .5 0 ,等位基因频率为 :D0 .30 ,I0 .70。 EH组与对照组基因型和等位基因频率差异无显著性。结论　 ACE基因 I/ D多态性与山西籍汉族人群高血压病无关联 ,三条引物法可以 1次完成 ACE基因分型

Insertion /deletion polymorphism of ACE gene in essential hypertension in Han nationality in Shanxi province

Objective There is insertion/deletion (I/D) polymorphism in human angiotension converting enzyme (ACE) gene.The method developed by Rigat might sometimes mistype ID heterozygotes as DD homozygotes.The purpose of this study is using triple primer method to detect the ACE genotyping in Shanxi,and the association of the ACE gene I/D polymorphism with essential hypertension (EH).Method Forty-one Shanxi nuclear families including 123 patients with EH were genotyped by triple primer method;98 normotensive subjects were genotyped by triple primer method. Result There was no significant difference in frequencies of both genotypes and alleles between EH group and normotensive group.Conclusion D allel of ACE is not associated with EH in hypertensive population in Han nationality Shanxi Province.