丹参中酚酸类成分在不同工艺条件下转化关系

目的:研究提取时间、碱处理、转化时间等因素对丹参中酚酸类成分水解转化规律影响。方法:采用高效液相色谱法,分别考察了丹参饮片在不同加热时间、p H及反应时间下的丹参酚酸类成分峰面积变化规律。结果:在加热的6 h内,丹酚酸B在受热30 min峰面积最大,随后丹酚酸B开始发生降解,丹参素、原儿茶醛、丹酚酸A是终产物。丹酚酸A进一步在碱性条件下又发生降解,生成丹酚酸C和异丹酚酸C一对同分异体。结论:揭示了丹参在受热、p H条件下中丹酚酸类成分的转化规律,为丹参在中药制剂生产和临床应用中提供理论基础。

Transformation Relationship of Salvianolic Acids in Salviae Miltiorrhizae Radix et Rhizoma Under Heating,pH Conditions

Objective: To study the regularity of extraction time, pH of the phenolic acids in Salviae Miltiorrhizae Radix et Rhizoma. Method: Respectively investigates the Salviae Miltiorrhizae Radix et Rhizoma at different heating time, pH, and the reaction time of phenolic acids peak area change rule by High-performance liquid chromatography. Result: The content of salvianolic acid B was increasing and reached the maximum at 30 min,but it decreased from 1 to 6 h. Tanshinol,protocatechuic aldehyde, salvianolic acid A is the final product. Salvianolic acid A could be converted into Salvianolic acid C and iso-salvianolic acid C by alkaline hydolysis. Conclusion: Reveals transformation of phenolic acids under heating, pH conditions,providing theoretical support in future Chinese medicine production and clinical applications of Salviae Miltiorrhizae Radix et Rhizoma.