续断水提液对小鼠急性毒性和遗传毒性的影响

目的:探讨续断水提液对小鼠的急性毒性和遗传毒性。方法:采用最大耐受剂量法评价续断水提液对小鼠的急性毒性,观察小鼠体重及心脏、脾脏、肝脏、肾脏指数的变化;采用微核实验和单细胞凝胶电泳实验评价其遗传毒性,将40只小鼠随机分成空白组(ig 10 m L·kg~(-1)生理盐水),环磷酰胺组(第4天开始注射0.1 g·kg~(-1)环磷酰胺),续断水提液高、中、低剂量组(ig 32,16,8 g·kg~(-1)续断水提液)。各组小鼠给药5 d后处死并解剖,取骨髓细胞检测嗜多染红细胞的微核率;取胸腺、肝脏及脾脏细胞检测续断水提液对小鼠DNA的损伤。结果:续断水提液经口给药小鼠的最大给药量为80 g·kg~(-1),该剂量对小鼠体重及心脏、脾脏、肝脏、肾脏指数均无显著影响。中、低剂量组小鼠嗜多染红细胞的微核率与空白组比较无显著性差异;但续断高剂量组微核率明显高于空白组(P0.05)。高、中、低剂量组胸腺、脾脏、肝脏细胞尾部DNA百分率,尾矩略高于空白组,但均无显著性差异。结论:续断水提液对小鼠最大给药剂量为80 g·kg~(-1),属无毒级;中和低剂量续断水煎液对小鼠无遗传毒性作用,高剂量续断水提液可诱发小鼠骨髓嗜多染红细胞微核率升高,存在潜在的遗传毒性。

Acute Toxicity and Genotoxicity of Dipsaci Radix Water Extract Liquid in Mice

Objective: To explore the acute toxicity and genotoxicity of Dipsaci Radix in mice. Method: The acute toxicity of Dipsaci Radix in mice was evaluated by the maximum tolerated dose method, and the changes of body weight, heart,spleen,liver,and kidney indexes were observed. Its genotoxicity was evaluated by micronucleus test and single cell gel electrophoresis test. 40 mice were divided into five groups randomly:blank group (normal saline of 10 mL · kg^-1 by gavage), cyclophosphamide group (0.1 g · kg^-1 cyclophosphamide was injected since the fourth day),and Dipsaci Radix water extract liquid high dose group, middle dose group and low dose group (32,16 and 8 g · kg^-1 by gavage). 5 days latter,all of the mice were sacrificed and anatomized.The bone marrow cells were taken to detect the micronucleus rate of polychromatic erythrocytes,and the DNA damages of thymus,liver and spleen were detected by single cell gel electrophoresis test. Result: The maximum tolerated dose was 80 g · kg^-1 for Dipsaci Radix water extract liquid by oral taking in mice,and such dose had no significant effect on body weight and heart,spleen,liver,and kidney indexes. Compared with blank group, there was no significant difference in micronucleus rate of polychromatic erythrocytes in middle and low dose groups, but the micronucleus rate of polychromatic erythrocytes in high dose group was significantly higher than that in blank group (P<0.05).The tail DNA percent and tail moment of thymus,liver and spleen in high, middle and low dose groups were a little higher than those in blank group, but with no significant difference. Conclusion: The maximum tolerated dose of Dipsaci Radix water extract liquid is 80 g ·kg^-1 in mice, with no acute toxicity on mice. Middle and low doses of Dipsaci Radix water extract liquid have no genotoxicity on mice, while high dose of Dipsaci Radix has increased the micronucleus rate of polychromatic erythrocytes, showing potential genotoxicity on mice.