白花蛇舌草治疗大鼠胶原诱导性关节炎的实验研究

目的探讨白花蛇舌草(HD)对胶原诱导性关节炎(CIA)大鼠模型的抗炎效果及其免疫学机制。方法首先建立Wistar大鼠CIA模型,造模成功后随机分为正常对照组、CIA模型对照组、白花蛇舌草正常量组、白花蛇舌草高剂量组、雷公藤组并给予相应处理。每周观察各给药组的关节炎指数,于第6周处死大鼠检测各组血清的TNF-α、IL-6的水平。结果 HD组大鼠关节炎指数显著降低(P<0.05或0.01);免疫后第6周,2个HD组大鼠血清TNF-α及IL-6的水平较CIA模型组明显降低(P<0.05),与雷公藤组当。结论 HD能够缓解CIA大鼠症状、控制炎症,其机制是抑制TNF-α和IL-6的水平。

Study on heduotis diffusa in treating rat with collagen-induced arthritis

Objective It is to approach the effect and immunological mechanism of hedyotis diffusa(HD) on treatment of rats with collagen-induced arthritis(CIA).Methods CIA model of Wistar rats were induced,then were randomly divided into five groups: normal control group,CIA model group,normal-doses HD group,high-dose HD group,tripterygium group,and were treated with homologous drugs.The foot diameter,the arthritic index of all rats at different time points were observed and measured.At the 6th week,all rats were killed to measure the changes of serum cytokine levels including TNF-α,IL-6.Results The arthritis indexes in both HD groups were significant reduced(P<0.05 or 0.01).The serum levels of TNF-α and IL-6 in both HD groups were significant decreased than CIA model group since 6th week(P<0.05),and it was similar to tripterygium group.Conclusion HD can release symptom of CIA rats and inhibit inflammation,the mechanism is that HD can decrease the expression of TNF-α and IL-6.