弥漫性系膜增生伴局灶节段性肾小球硬化大鼠模型的建立

目的：建立弥漫性系膜增生伴局灶节段性肾小球硬化动物模型。方法：对SD大白鼠行腹部部正中切口右肾摘除术，术后7d,30d分别尾静脉注射阿霉素4mg/kg,3mg/kg，第70d制成模型，结果：该模型临床上表现为高度浮肿，大量蛋白尿、低蛋白血症、高脂血症及肾功能损害；病理学检查，计算机图象分析及免疫组织化学染色显示，肾小球主要表现为系膜基质弥漫性中至重度增生，系膜细胞轻度增生，其中约22％的肾小球出现节段性硬化，纤维化生长因子血不板源性生长因子－B（PDGF－B）和成纤维细胞生长因子－b(bFGF)的表这均增加，表明肾小球正处于由增生向硬化转化转化的过渡阶段。结论：本模型的建立为实验研究肾小球疾病由系膜增生向硬化转化的机制及防治措施创造了条件。

Establishment of Model of Diffuse Mesangioproliferation Complicated With Focal Segmentally Glomerulosclerosis Model of Rat

Objective To establish a animal model for diffusion mesangioproliferation complicating focal segmentally glomerulosclerosis.Methods The models were established in the seventieth day by repetitive injection of 4mg/kg and 3mg/kg adriamycin to the SD rats in the 7th and 30th day after removal of their right kidneys.Results Serious edema, profuse albuminuria, hypoalbuminemia, hyperlipemia and renal failure were presented. Simultaneously, diffuse medium- or serious-grade proliferation of mesangial matrix, slight-grade proliferation of mesangial cells, segmental sclerosis of 22% of glomeruli, and overexpression of PDGF-B and bFGF in glomeruli were showed by pathologic examination, computerized image analysis and immunohistochemistry staining.Conclusions The models established in the transition stage from mesangioproliferation to glomerulosclerosis are helpful to study mechanisms and take measures for prevention and cure of chronic glomerulonephritis.