卵巢癌细胞中ERK1／2的表达及其与细胞凋亡的关系

目的研究细胞外信号调节激酶ERK1／2在卵巢癌细胞SKOV3中的表达及其与细胞相关凋亡蛋白的关系。方法用MTY检测17β-E2对细胞的增殖情况，用MEK1／2通路抑制剂U0126在不同浓度、不同时间处理细胞后，RT—PCR检测细胞ERK1mRNA、ERK2mRNA、caspase．3mRNA的表达。结果浓度为10^-5mol／L17β-E2作用1h可显著促进细胞增殖（5．7±0．23）％]，与对照组（3．5±0．45）％]相比，P=0．000。ERK1／2在卵巢癌中高表达，随着U0126处理细胞浓度的增加及时间的延长，ERK1的表达逐渐降低，与对照组相比，P值分别为0．176、0．009、0．002、0．000；ERK2的表达逐渐降低，与对照组相比，P值分别为0．010、0．000、0．000、0．000；caspase-3的表达逐渐增加，与对照组相比，P值分别为1．000、0．290、0．090、0．003。结论ERK1／2与卵巢癌的发生发展有关，抑制ERK1／2的表达可抑制细胞增殖，增加细胞凋亡。

The expression of ERK1/2 in ovarian carcinoma cell and its correlation with apoptosis

Objective To investigate the expression of extracellular signal-regulated kinasel/2 （ERK1/2） in ovarian carcinoma cell and its correlation with apoptosis. Methods The proliferation was assayed by MTr when the cells were exposed to 17β-E2. After the cell was treated with U0126 which is MEK suppressor,the expression of caspase-3, ERK1, ERK2 mRNA in ovarian carcinoma cell were detected by RT-PCR in different concentration and different time. Results The cell proliferative rate was significantly higher （5.7 ± 0.23 ） % ] after stimulation with 17β-E2 at 1 × 10^-6 mol/L for 60 rain than control （3.5 ±0. 45）% ] （P =0. 000）. ERK1/2 was highly expressed, after the cell were treated with U0126 ,along with increasing of time and concentration,the expression of ERK1 was increasingly reduction, compared to control group, P = 0. 176,0. 009,0. 002,0. 000 ; the expression of ERK2 was increasingly reduction too, compared to control group, P = 0. 010,0. 000,0. 000,0. 1300; while the expressior± of caspase-3 were increasingly highly than control, P = 1. 000, 0. 290, 0. 090, 0. 003. Conclusion ERK1/2 is participate in the occurrence and development of ovarian cancer. When ERK1/2 expression is inhibited, cell proliferation is inhibited,while cell apoptosis is increased.