RP-HPLC同时测定菝葜中山柰酚和槲皮素的含量

 目的建立一种RP-HPLC测定菝葜山柰酚和槲皮素含量的方法,并测定其不同产地的含量。方法采用LichrospherC₁₈柱,水-乙腈-磷酸-三乙胺(65∶35∶0.27∶0.45)作流动相,流速0.7 mL·min^-1,二级管阵列检测器(DVD),检测波长365 nm。结果槲皮素,山柰酚与相邻杂质能很好分离,其分离度分别为1.96和5.32,理论塔板数以槲皮素峰和山柰酚峰计算,分别为5 121和6 673;在5～100 mg·L^-1内峰面积与其浓度均呈良好的线性关系(r=0.999 4,0.999 6),样品回收率分别为97.58%和105.67%;两成分含量以湖南张家界的最高而湖北赤壁最低。结论该方法精密度、重现性和分离效果好,分析快速准确,适合于该药材及提取物中山柰酚和槲皮素含量的测定,不同产地两成分均存在一定的差别。

Determination of Kaempferol and Quercetin in Smilax china by RP-HPLC

OBJECTIVE To establish a RP-HPLC method for the determination of kaempferol and quercetin in Smilax china L.,and to investigate their contents in different places.METHODS The determination was performed on a Lichrospher C₁₈ column(4.6 mm×250 mm,5 μm).The detection wavelength was at 365 nm,with the mobile phase of Water-Acetonitrile-Phosphoric-Triethylamine(65∶35∶0.27∶0.45) at the flow rate of 0.7 mL·min^-1.RESULTS Kaempferol,quercetin and impurities were separated.The theoretical plate numbers of kaempferol and quercetin were 5 121 and 6 673,the resolution between kaempferol,quercetin and impurities were 1.96 and 5.32,respectively.All the parameters were suitable for determination.Both of kaempferol and quercetin showed a good linearity over the range of 5～100 mg·L^-1(r=0.999 4,0.999 6),respectively,the average recoveries of kaempferol and quercetin were 105.67% and 87.58%,respectively.Their contents were highest in Zhang jia jie,Hunan and lowest in Chibi,Hubei.CONCLUSION The RP-HPLC method shows a good separation,reproducity and accuracy.It will be used for determining kaempferol and quercetin in herbs and extracts.The contents of kaempferol and quercetin are different in Smilax china L.from different places.