On the analysis of 1-nitronaphthalene, 1-nitropyrene and 6-nitrochrysene in cigarette smoke

A method was developed for the analysis of 1-nitro-naphthalene,1-nitropyrene, and 6-nitrochrysene in main stream cigarettesmoke, using [¹⁴C]1-nitronaphthalene, [¹⁴C]1-nitropyrene, and[¹⁴C]6-nitrochrysene as tracers and internal standards. Cigarettesmoke condensate was collected and the appropriate fractionscontaining the labelled standards were obtained by a seriesof solvent partitions and column chromatography. Recovery ofinternal standards ranged from 60 to 70%. The fractions wereanalyzed by capillary gas chromatography with electron capturedetection and by combined capillary gas chromatography –mass spectrometry. 1-Nitronaphthalene (<10ng/cigarette),1-ni-tropyrene (<10ng/cigarette), and 6-nitrochrysene (<1ng/cigarette)were not detected in the mainstream smoke of the Universityof Kentucky 1R1 cigarette, a US commercial 85 mm non-filtercigarette, or a French commercial 70 mm non-filter cigarette.