基于16S rDNA基因测序技术分析不同身体质量指数人群的肠道菌群结构差异

目的:探究不同身体质量指数(BMI)人群肠道菌群的丰度及物种差异性.方法:将115例受试人群按BMI分为正常组、超重组和肥胖组,收集受试者粪便,运用16SrDNA基因测序技术结合生物信息分析,比较不同BMI受试者肠道菌群组成和丰度差异.结果:Alpha多样性分析可见超重组和肥胖组群落丰度和多样性较正常组低;不同分组的肠...

Analysis of intestinal flora structure differences in different BMI populations based on 16S rDNA gene sequencing technology

Objective:To explore the abundance and species differences of intestinal flora in different body mass index(BMI)populations.Methods:According to BMI,115 subjects were divided into a normal group,overweight group and obesity group.The feces of subjects were collected,and the composition and abundance of the intestinal flora of subjects with different BMI were compared by using 16 S rDNA gene sequencing technology and biological information analysis.Results:The results of alpha diversity analysis showed that the community richness and diversity of super recombination and obesity group were lower than that of normal group.The composition of intestinal flora in different groups was significantly different at OUT level.Firmicutes,Bacteroides,actinobacteria and proteobacteria are the dominant bacteria in three groups of intestinal flora;Ehrlich-Shigella and Rumen Coccus were significantly different from each other(P<0.05).Compared with the normal group,the differences of Bacteroides,Lachnoclostridium and Collinia in the obesity group were significant(P<0.05,P<0.01).Conclusion:The composition of intestinal flora in different BMI populations is significantly different,and the diversity of intestinal flora in obese and overweight people is significantly reduced.The abundance of Bacteroides,Lachnoclostridium and Collins varied obviously,which suggested that they might be involved in the accumulation of body fat.Regulating the diversity and abundance of intestinal microflora may be one of the effective ways to prevent and treat intestinal micro-ecological related diseases.