黄芪汤对12C6+辐射脑损伤模型鼠细胞因子、外周血象及Survivin蛋白表达的影响

目的:观察黄芪汤对12C6+辐射脑损伤模型鼠脾脏细胞因子、外周血象及Survivin蛋白表达的影响,以揭示其防辐射分子机制.方法:50只SPF级Wistar大鼠随机分为正常对照组,单纯辐射模型组,黄芪汤高、中、低剂量组,每组10只.黄芪汤高、中、低剂量组分别给予黄芪汤18.0、9.0、4.5g·kg 1·d-1,灌胃2...

Effects of Huangqi Decoction on spleen cell factor,peripheral blood and Survivin protein expression in rats with C radiation brain damage

Objective:To observe the effects of Huangqi Decoction on cell factor,peripheral blood and Survivin protein expression in rats with ¹²C⁶⁺radiation brain damage,and reveal its molecular mechanism of radiation protection.Methods:Fifty Wistar rats were randomly divided into five groups:normal control group,radiation alone model group,highdose,middle-dose and low-dose Huangqi Decoction group.After 7 days of routine feeding,the high-dose,middle-dose and low-dose Huangqi Decoction group were given Huangqi Decoction(18.0,9.0,4.5 g·kg^-1·d^-1)by gavage for one weeks in advance.The normal control group and the radiation alone groups were given the equal normal saline.After gavage for 7 d,rats of all groups except the control group were radiated by 4 Gy ¹²C⁶⁺ion once.After radiation for 7 d,all groups of rats were executed.The pathomorphism changes of renal tissue were observed by HE,the contens of IL-1β,IL-2 and IL-6 in spleen tissue were checked by ELISA,the number changes of peripheral blood cells were detected by hematology analyzer,the amount of mRNA and protein expression of Survivin in kidney was examined with RT-PCR and Western Blot.Results:Compared with the normal control group,the body weight and the indexes of thymus and spleen significantly decreased,the IL-2 level in spleen significantly reduced,the number of WBC and RBC in peripheral blood significantly cut down,the gene and protein of Survivin in kidney tissue significantly decreased,whereas the levels of IL-1βand IL-6 in spleen significantly raised(P<0.05,P<0.01).The mesangial cells proliferated obviously,interstitial vessels of renal tubules dilated and congested obviously,the lumen of renal tubules was narrow and irregular in the radiation alone model group.Compared with the radiation alone model group,the body weight,IL-2 level in spleen and the number of PLT in peripheral blood obviously raised in high-dose huangqi decoction group,yet the levels of IL-1βand IL-6 in spleen significantly decreased in high-dose Huangqi Decoction group,the PLT number obviously decreased in low-dose,middle-dose Huangqi Decoction group,the gene and protein of Survivin in kidney tissue,the number of WBC and RBC in peripheral blood significantly increased in middle-dose,high-dose Huangqi Decoction group,the indexes of thymus and spleen obviously raised in Huangqi Decoction intervention group(P<0.05,P<0.01).The proliferation of mesangial cells was improved and the contour of renal tubules was clear in high-dose Huangqi Decoction group.Conclusion:Huangqi Decoction has a certain protective effect on the organ damage caused by ¹²C⁶⁺radiation brain induced bystander effect in vivo.Huangqi Decoction reduced the organ injury in rats with ¹²C⁶⁺radiation brain through immunity enhancement,inhibition of apoptosis in renal tissue and the hematopoietic system rebuilt and body weight regain.