蛇床子素对卵巢癌SKOV3细胞凋亡的影响

目的:探讨蛇床子素对卵巢癌SKOV3细胞增殖与凋亡的影响以及其潜在的机制。方法:不同浓度蛇床子素作用于卵巢癌SKOV3细胞,采用MTT法检测蛇床子素对细胞增殖的影响;Hoechst 33258染色和流式细胞术检测蛇床子素对细胞凋亡的影响;实时荧光定量PCR(qRT-PCR)和Western Blot检测Bax、Bcl-2 mRNA和蛋白的表达水平。结果:蛇床子素明显抑制SKOV3细胞的增殖;蛇床子素干预后,有典型的凋亡小体形成,SKOV3细胞的凋亡率随着药物浓度的增加而增加,80mg/L处理组细胞的凋亡率达到(24.90±5.35)%;qRT-PCR和Western Blot结果显示各浓度蛇床子素对SKOV3细胞促凋亡因子Bax mRNA和蛋白水平表达均有上调作用,而抗凋亡因子Bcl-2mRNA和蛋白表达均下调。结论:蛇床子素能够抑制卵巢癌SKOV3细胞的增殖并诱导其凋亡,其机制可能与其下调抗凋亡因子Bcl-2和上调促凋亡因子Bax的表达有关。

Effects of osthole on apoptosis of ovarian cancer SKOV3 cells

Objective:To investigate the effects of osthole on proliferation and apoptosis of ovarian cancer SKOV3 cells and its potential mechanism.Methods:SKOV3 cells were treated with different concentrations of osthole.The effects of osthole on cell proliferation was examined by MTT assay.Hoechst 33258 and flow cytometry were used to detect the effects of osthole on apoptosis.The expression levels of Bax and Bcl-2 mRNA and protein were detected by quantitative real-time PCR(qRT-PCR)and Western Blot.Results:Osthole significantly inhibits the proliferation of SKOV3 cells.After osthole intervention,typical apoptotic bodies were formed.The apoptosis rate of SKOV3 cells increased with the increase of drug concentration.The apoptosis rate of cells treated with 80 mg/L even reached(24.90±5.35)%.The results of qRT-PCR and Western Blot showed that the expression of proapoptotic factor Bax mRNA and protein were increased,while the mRNA and protein expression of antiapoptotic factor Bcl-2 were down-regulated.Conclusion:Osthole can inhibit the proliferation of ovarian cancer SKOV3 cells and induce its apoptosis,which may be related to the down-regulation of anti-apoptotic factor Bcl-2 and up-regulation of the expression of Bax.